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Product Info Summary
| SKU: | EK1689 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Haptoglobin ELISA Kit PicoKine®
View all Haptoglobin ELISA kits
SKU/Catalog Number
EK1689
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Haptoglobin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Hp in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Haptoglobin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1689)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: V19-N347
Sensitivity
<20 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK1689 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1689 is reactive to Hp in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1689 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Haptoglobin
Haptoglobin (HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. A major function of haptoglobin is to bind hemoglobin (Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. In clinical settings, the haptoglobulin assay is used to screen for and monitor intravascular hemolytic anemia .
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1689-CAP | Anti-Mouse Hp Pre-coated 96-well strip microplate | 1 |
| EK1689-ST | Mouse Hp Standard | 2 vials, 100 ng/tube |
| EK1689-DA | Mouse Hp Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.010 | 0.039 | 0.072 | 0.120 | 0.216 | 0.422 | 0.923 | 1.874 |
Data Example Images
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Mouse Haptoglobin PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Haptoglobin, Dilution ratio of 1:5000, concentration in serum and plasma is around 74 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Haptoglobin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 2678 | 12849 | 42168 | 2657 | 13883 | 41875 |
| Standard deviation | 200.85 | 976.52 | 1981.89 | 207.24 | 1082.87 | 2093.75 |
| CV (%) | 7.5% | 7.6% | 4.7% | 7.8% | 7.8% | 5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Haptoglobin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 2678 | 2633 | 2766 | 2754 | 2707 | 54.78 | 2% |
| Sample 2 | 12849 | 14795 | 13542 | 15077 | 14065 | 909.56 | 6.4% |
| Sample 3 | 42168 | 46553 | 47581 | 44731 | 45258 | 2055.43 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For Hp (Source: Uniprot.Org, NCBI)
Gene Name
Hp
Full Name
Haptoglobin
Weight
45.205kDa
Superfamily
peptidase S1 family
Alternative Names
Haptoglobin; Zonulin; Haptoglobin alpha chain; Haptoglobin beta chain; Hp HP BP2ALPHA2, HPA1S, HP haptoglobin haptoglobin|binding peptide|haptoglobin alpha(1S)-beta|haptoglobin alpha(2FS)-beta|haptoglobin, alpha polypeptide|haptoglobin, beta polypeptide|zonulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Hp, check out the Hp Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Hp: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Haptoglobin ELISA Kit PicoKine® (EK1689)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Mouse Haptoglobin ELISA Kit PicoKine®
Question
Have you tested mouse plasma samples with EK1689?
Verified customer
Asked: 2022-06-14
Answer
Yes, we tested the Mouse Haptoglobin ELISA Kit PicoKine® (EK1689) on mouse plasma samples. The dilution ratio is 1:5000, concentration in mouse plasma is around 74ug/ml.
Boster Scientific Support
Answered: 2022-06-16
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-03-06
Question
Q: is it okay to use citrate plasma as samples in Mouse Haptoglobin Picokine® ELISA Kit (Catalog # EK1689)?
Verified Customer
Verified customer
Asked: 2020-03-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Haptoglobin causing disruption of its protein structure. Haptoglobin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Haptoglobin ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-03-01
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Haptoglobin in Mouse serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-08-31
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Haptoglobin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-08-31
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-08-03
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Haptoglobin using the EK1689 Mouse Haptoglobin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-03-22
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2017-03-22
Question
Q: how to analyze ELISA data? I have obtained Haptoglobin level in serum.
T. Chakrabarti
Verified customer
Asked: 2016-01-14
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-01-14
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2014-09-14
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-09-14
