Anti-SHROOM1 Antibody Picoband™

Product Name

Anti-SHROOM1 Antibody Picoband™

View all Shroom1 Antibodies

SKU/Catalog Number

A12817-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SHROOM1 Antibody Picoband™ catalog # A12817-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SHROOM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12817-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SHROOM1 recombinant protein (Position: R371-D807).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A12817-1 is reactive to Shroom1 in Human, Mouse, Rat

Applications

A12817-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

91 kDa

Calculated molecular weight

Background of Shroom1

SHROOM family members play diverse roles in the development of the nervous system and other tissues. SHROOM1 is a PDZ domain-containing actin-binding protein that is required for neural tube morphogenesis. It facilitates neurulation by regulating the morphology of neurepithelial cells via the apical positioning of an actomyosin network in the neurepithelium. SHROOM1 is also expressed in pulmonary arterial smooth muscle cells and its expression is significantly decreased in mouse and human models of pulmonary arterial hypertension, suggesting that there may be a link between SHROOM1 expression and pulmonary arterial hypertension.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SHROOM1 using anti-SHROOM1 antibody (A12817-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM1 antigen affinity purified polyclonal antibody (Catalog # A12817-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM1 at approximately 91 kDa. The expected band size for SHROOM1 is at 91 kDa.

Gene/Protein Information For Shroom1 (Source: Uniprot.org, NCBI)

Alternative Names

Apical Protein 2; APXL2; KIAA1960; Protein Shroom1; Shroom Family Member 1 Shroom1|A, Apx, Shr, Shrm1|shroom family member 1|protein Shroom1

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