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Product Info Summary
| SKU: | PB9675 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SHP2/PTPN11 Antibody Picoband®
View all SHP-2/PTPN11 Antibodies
SKU/Catalog Number
PB9675
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SHP2/PTPN11 Antibody Picoband® catalog # PB9675. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHP2/PTPN11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9675)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SHP2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9675 is reactive to PTPN11 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
68 kDa
Calculated molecular weight
68436 MW
Background of SHP-2/PTPN11
PTPN11 (Tyrosine-protein phosphatase non-receptor type 11), also known as protein-tyrosine phosphatase 1D (PTP-1D), protein-tyrosine phosphatase 2C (PTP-2C), TYROSINE PHOSPHATASE SHP2 (SHP2), BPTP3, SH-PTP2, SHP-2, SH-PTP3, is an enzyme that in humans is encoded by the PTPN11 gene. PTPN11 is a member of the protein tyrosine phosphatase (PTP) family. The open reading frame consists of 1,779 nucleotides potentially encoding a protein of 593 amino acids with a predicted molecular mass of 68 kD. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9675 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, Rat Cardiac Muscle Tissue, Mouse Kidney Tissue, HELA Whole Cell, SW620 Whole Cell, HEPG2 Whole Cell, JURKAT Whole Cell
IHC: mouse cardiac muscle tissue, rat skeletal muscle tissue, human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SHP2 using anti-SHP2 antibody (PB9675).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Brain Tissue Lysate at 50ug,
Lane 2: Mouse Brain Tissue Lysate at 50ug,
Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug,
Lane 4: Mouse Kidney Tissue Lysate at 50ug,
Lane 5: HELA Whole Cell Lysate at 40ug,
Lane 6: SW620 Whole Cell Lysate at 40ug,
Lane 7: HEPG2 Whole Cell Lysate at 40ug,
Lane 8: JURKAT Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2 antigen affinity purified polyclonal antibody (Catalog # PB9675) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68 kDa. The expected band size for SHP2 is at 68 kDa.
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Figure 2. IHC analysis of SHP2 using anti-SHP2 antibody (PB9675).
SHP2 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SHP2 Antibody (PB9675) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SHP2 using anti-SHP2 antibody (PB9675).
SHP2 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SHP2 Antibody (PB9675) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SHP2 using anti-SHP2 antibody (PB9675).
SHP2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SHP2 Antibody (PB9675) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PTPN11 (Source: Uniprot.org, NCBI)
Gene Name
PTPN11
Full Name
Tyrosine-protein phosphatase non-receptor type 11
Weight
68436 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Tyrosine-protein phosphatase non-receptor type 11;3.1.3.48;Protein-tyrosine phosphatase 1D;PTP-1D;Protein-tyrosine phosphatase 2C;PTP-2C;SH-PTP2;SHP-2;Shp2;SH-PTP3;PTPN11;PTP2C, SHPTP2; PTPN11 BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2 protein tyrosine phosphatase non-receptor type 11 tyrosine-protein phosphatase non-receptor type 11|SH2 domain-containing protein tyrosine phosphatase 2|protein-tyrosine phosphatase 1D|protein-tyrosine phosphatase 2C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPN11, check out the PTPN11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPN11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHP2/PTPN11 Antibody Picoband® (PB9675)
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No publications found for PB9675
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Customer Q&As
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2 Customer Q&As for Anti-SHP2/PTPN11 Antibody Picoband®
Question
Has PB9675 been tested for IP application?
Verified customer
Asked: 2019-10-09
Answer
Unfortunately, our lab has not tested the Anti-SHP2/PTPN11 Antibody Picoband™ (PB9675) for IP application.
Boster Scientific Support
Answered: 2019-10-09
Question
We are currently using anti-SHP2/PTPN11 antibody PB9675 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-SHP2/PTPN11 antibody (PB9675) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
