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Product Info Summary
| SKU: | M00099 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ras NRAS Rabbit Monoclonal Antibody
SKU/Catalog Number
M00099
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ras NRAS Rabbit Monoclonal Antibody catalog # M00099. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ras NRAS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00099)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOFE-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ras
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00099 is reactive to NRAS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated molecular weight
21229 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00099 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: SH-SY5Y cell
Validation Images & Assay Conditions
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Western blot analysis of Ras expression in SH-SY5Y cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NRAS (Source: Uniprot.org, NCBI)
Gene Name
NRAS
Full Name
GTPase NRas
Weight
21229 MW
Superfamily
small GTPase superfamily
Alternative Names
GTPase NRas;Transforming protein N-Ras;NRAS;HRAS1; NRAS ALPS4, CMNS, N-ras, NCMS1, NS6, NRAS NRAS proto-oncogene, GTPase GTPase NRas|N-ras protein part 4|neuroblastoma RAS viral (v-ras) oncogene homolog|neuroblastoma RAS viral oncogene homolog|transforming protein N-Ras|v-ras neuroblastoma RAS viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NRAS, check out the NRAS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NRAS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ras NRAS Rabbit Monoclonal Antibody (M00099)
Hello CJ!
M00099 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li M, Liu W, Zhu Yf, Chen Yl, Zhang Bz, Wang R. Acta Oncol. 2006;45(8):1115-9. Correlation Of Cox-2 And K-Ras Expression To Clinical Outcome In Gastric Cancer.
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Customer Q&As
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5 Customer Q&As for Anti-Ras NRAS Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemia using anti-Ras Rabbit Monoclonal antibody M00099. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-04
Question
Would M00099 anti-Ras Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
As indicated on the product datasheet, M00099 anti-Ras Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-22
Question
I was wanting to use your anti-Ras Rabbit Monoclonal antibody for IP for rat leukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukemia identification?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
It shows on the product datasheet, M00099 anti-Ras Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-25
Question
Do you have a BSA free version of anti-Ras Rabbit Monoclonal antibody M00099 available?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Ras Rabbit Monoclonal antibody M00099 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-20
Question
Please see the WB image, lot number and protocol we used for leukemia using anti-Ras Rabbit Monoclonal antibody M00099. Please let me know if you require anything else.
C. Thomas
Verified customer
Asked: 2015-04-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-03
