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Product Info Summary
| SKU: | A00150 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-SHP-2 (Ab-542) PTPN11 Antibody
View all SHP-2/PTPN11 Antibodies
SKU/Catalog Number
A00150
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SHP-2 (Ab-542) PTPN11 Antibody (Catalog # A00150). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHP-2 (Ab-542) PTPN11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00150)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 540~544 (H-E-Y-T-N) derived from Human SHP-2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00150 is reactive to PTPN11 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
33429 MW
Background of SHP-2/PTPN11
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
Ferjoux G, et al. (2003) Mol Biol Cell. 2003; 14(9): 3911-3928.
Shi ZQ, et al. (2000) Mol Cell Biol ; 20(5): 1526-1536.
Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96(17): 9677-9682
Manes S, et al. (1999) Mol Cell Biol ; 19(4): 3125-3135.
Oh ES, et al. (1999) Mol Cell Biol; 19(4): 3205-3215.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00150 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 72kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTPN11 using anti-PTPN11 antibody (A00150).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN11 antigen affinity purified polyclonal antibody (Catalog # A00150) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PTPN11.
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Immunofluorescence staining of methanol-fixed Hela cells using SHP-2(Ab-542) Antibody #A00150.
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Figure 2. IHC analysis of PTPN11 using anti-PTPN11 antibody (A00150).
PTPN11 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PTPN11 Antibody (A00150) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PTPN11 (Source: Uniprot.org, NCBI)
Gene Name
PTPN11
Full Name
Tyrosine-protein phosphatase non-receptor type 11
Weight
33429 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
G1/S-specific cyclin-D1;Ccnd1;Cyl-1; PTPN11 BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2 protein tyrosine phosphatase non-receptor type 11 tyrosine-protein phosphatase non-receptor type 11|SH2 domain-containing protein tyrosine phosphatase 2|protein-tyrosine phosphatase 1D|protein-tyrosine phosphatase 2C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPN11, check out the PTPN11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPN11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHP-2 (Ab-542) PTPN11 Antibody (A00150)
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No publications found for A00150
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