Product Info Summary
SKU: | A30482 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-SFRS5 Antibody
SKU/Catalog Number
A30482
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SFRS5 Antibody (Catalog# A30482). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFRS5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30482)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SFRS5. Immunogen sequence location: 71-120
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30482 is reactive to SRSF5 in Human, Mouse, Rat
Applications
A30482 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
31264 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
![SFRS5 primary antibodies A30482 IF testing SFRS5 primary antibodies A30482 IF testing](https://www.bosterbio.com/media/catalog/product/S/F/SFRS5-primary-antibodies-A30482-IF-testing.jpg)
Click image to see more details
Immunofluorescence analysis of MCF7 cells, using SFRS5 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For SRSF5 (Source: Uniprot.org, NCBI)
Gene Name
SRSF5
Full Name
Serine/arginine-rich splicing factor 5
Weight
31264 MW
Superfamily
splicing factor SR family
Alternative Names
Delayed-early protein HRS; HRSSFRS5; serine/arginine-rich splicing factor 5; Splicing factor, arginine/serine-rich 5Pre-mRNA-splicing factor SRP40; SRP40SR splicing factor 5 SRSF5 HRS, SFRS5, SRP40 serine and arginine rich splicing factor 5 serine/arginine-rich splicing factor 5|SR splicing factor 5|delayed-early protein HRS|pre-mRNA-splicing factor SRP40|splicing factor, arginine/serine-rich 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SRSF5, check out the SRSF5 Infographic
![SRSF5 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SRSF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFRS5 Antibody (A30482)
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No publications found for A30482
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Customer Q&As
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3 Customer Q&As for Anti-SFRS5 Antibody
Question
We are currently using anti-SFRS5 antibody A30482 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The anti-SFRS5 antibody (A30482) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-24
Question
Will A30482 anti-SFRS5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
As indicated on the product datasheet, A30482 anti-SFRS5 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-19
Question
I was wanting to use your anti-SFRS5 antibody for IF for rat left lobe of thyroid gland on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat left lobe of thyroid gland identification?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
It shows on the product datasheet, A30482 anti-SFRS5 antibody has been tested for IF on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat left lobe of thyroid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16