Product Info Summary
SKU: | A11740 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SFRS17A AKAP17A Antibody
SKU/Catalog Number
A11740
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SFRS17A AKAP17A Antibody (Catalog # A11740). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFRS17A AKAP17A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11740)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human SFRS17A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11740 is reactive to AKAP17A in Human, Mouse
Reconstitution
Calculated molecular weight
80735 MW
Background of XE7
Splice factor regulating alternative splice site selection for certain mRNA precursors. Mediates regulation of pre-mRNA splicing in a PKA-dependent manner.
Ellison J.W., Hum. Mol. Genet. 1:691-696(1992).
Voland J.R., Proc. Natl. Acad. Sci. U.S.A. 89:10425-10429(1992).
Ross M.T., Nature 434:325-337(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11740 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from NIH-3T3 cells, using SFRS17A antibody A11740.
Protein Target Info & Infographic
Gene/Protein Information For AKAP17A (Source: Uniprot.org, NCBI)
Gene Name
AKAP17A
Full Name
A-kinase anchor protein 17A
Weight
80735 MW
Alternative Names
A-kinase anchor protein 17A;AKAP-17A;721P;B-lymphocyte antigen;Protein XE7;Protein kinase A-anchoring protein 17A;PRKA17A;Splicing factor, arginine/serine-rich 17A;AKAP17A;CXYorf3, DXYS155E, SFRS17A, XE7; AKAP17A 721P, AKAP-17A, CCDC133, CXYorf3, DXYS155E, PRKA17A, SFRS17A, XE7, XE7Y A-kinase anchoring protein 17A A-kinase anchor protein 17A|A kinase (PRKA) anchor protein 17A|B-lymphocyte surface |protein kinase A-anchoring protein 17A|pseudoautosomal gene XE7|splicing factor, arginine/serine-rich 17A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AKAP17A, check out the AKAP17A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AKAP17A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFRS17A AKAP17A Antibody (A11740)
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No publications found for A11740
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Customer Q&As
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4 Customer Q&As for Anti-SFRS17A AKAP17A Antibody
Question
We are currently using anti-SFRS17A antibody A11740 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
P. Thomas
Verified customer
Asked: 2019-12-04
Answer
The anti-SFRS17A antibody (A11740) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-04
Question
Can you help my question with product A11740, anti-SFRS17A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11740 anti-SFRS17A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-22
Question
See attached the WB image, lot number and protocol we used for placenta using anti-SFRS17A antibody A11740. Please let me know if you require anything else.
K. Singh
Verified customer
Asked: 2019-03-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-18
Question
Would A11740 anti-SFRS17A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
As indicated on the product datasheet, A11740 anti-SFRS17A antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-30