Click image to see more details
Product Info Summary
| SKU: | M03147-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SFRP4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03147-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-SFRP4 Rabbit Monoclonal Antibody catalog # M03147-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFRP4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03147-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
20S67
Isotype
IgG
Immunogen
A synthesized peptide derived from human SFRP4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03147-1 is reactive to SFRP4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
191615 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03147-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human Hela whole cell, human 293T whole cell, human HepG2 whole cell, human MCF-7 whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SFRP4 using anti-SFRP4 antibody (M03147-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP4 antigen affinity purified monoclonal antibody (Catalog # M03147-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP4 at approximately 50 kDa. The expected band size for SFRP4 is at 40 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SFRP4 (Source: Uniprot.org, NCBI)
Gene Name
SFRP4
Full Name
Secreted frizzled-related protein 4
Weight
191615 MW
Superfamily
secreted frizzled-related protein (sFRP) family
Alternative Names
Clathrin heavy chain 1;Clathrin heavy chain on chromosome 17;CLH-17;CLTC;CLH17, CLTCL2, KIAA0034; SFRP4 FRP-4, FRPHE, FRZB-2, PYL, sFRP-4 secreted frizzled related protein 4 secreted frizzled-related protein 4|frizzled protein, human endometrium|secreted frizzled-related protein 4; secreted frizzled-related protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SFRP4, check out the SFRP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SFRP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFRP4 Rabbit Monoclonal Antibody (M03147-1)
Hello CJ!
No publications found for M03147-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SFRP4 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-SFRP4 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
