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Product Info Summary
| SKU: | A04734-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SERPINB9 Antibody Picoband®
SKU/Catalog Number
A04734-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SERPINB9 Antibody Picoband® catalog # A04734-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SERPINB9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04734-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SERPINB9 recombinant protein (Position: D176-K313).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04734-1 is reactive to SERPINB9 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
42.404kDa
Background of SERPINB9
Serpin B9 is a protein that in humans is encoded by the SERPINB9 gene. This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04734-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human K562 whole cell, human placenta tissue, human Daudi whole cell, human Raji whole cell
ICC/IF: A549 cell
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SERPINB9 using anti-SERPINB9 antibody (A04734-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: human Daudi whole cell lysates,
Lane 4: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINB9 antigen affinity purified polyclonal antibody (Catalog # A04734-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINB9 at approximately 38 kDa. The expected band size for SERPINB9 is at 38,42 kDa.
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Figure 2. IF analysis of SERPINB9 using anti-SERPINB9 antibody (A04734-1).
SERPINB9 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SERPINB9 Antibody (A04734-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of K562 cells using anti-SERPINB9 antibody (A04734-1).
Overlay histogram showing K562 cells stained with A04734-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SERPINB9 Antibody (A04734-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For SERPINB9 (Source: Uniprot.org, NCBI)
Gene Name
SERPINB9
Full Name
Serpin B9
Weight
42.404kDa
Superfamily
serpin family
Alternative Names
C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR-10; G-protein coupled receptor 2; CCR10; GPR2 SERPINB9 CAP-3, CAP3, PI-9, PI9 serpin family B member 9 serpin B9|cytoplasmic antiproteinase 3|peptidase inhibitor 9|protease inhibitor 9 (ovalbumin type)|serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 9|serpin peptidase inhibitor, clade B (ovalbumin), member 9|serpin peptidase inhibitor, clade B, member 9|testicular tissue protein Li 180
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINB9, check out the SERPINB9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINB9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SERPINB9 Antibody Picoband® (A04734-1)
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