Click image to see more details
Product Info Summary
| SKU: | PA1779 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-cardiac Troponin C/TNNC1 Antibody
View all Troponin C (cardiac) Antibodies
SKU/Catalog Number
PA1779
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-cardiac Troponin C/TNNC1 Antibody catalog # PA1779. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-cardiac Troponin C/TNNC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1779)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human cardiac Troponin C, identical to the related rat and mouse sequences .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1779 is reactive to TNNC1 in Human, Mouse, Rat
Applications
PA1779 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
18 kDa
Calculated molecular weight
18403 MW
Background of Troponin C (cardiac)
Troponin C1 (TNNC1) (troponin C type 1 (slow)), also called TROPONIN C, SLOW-TWITCH SKELETAL MUSCLE or TNC, is a protein that in humans is encoded by the TNNC1 gene. Using a "monochromosomal" hybrid panel, the TNNC1 gene is mapped to chromosome 3; subsequent analysis of the Genebridge 4 radiation hybrid panel localized the gene to 3p in a region consistent with the earlier assignment. Circular dichroism measurements revealed changes in the secondary structures of the TNNC1 mutants A8V, C84Y, and D145E.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-cardiac Troponin C antibody, PA1779, Western blotting
All lanes: Anti cardiac Troponin C (PA1779) at 0.5ug/ml
WB: Rat Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 18KD
Observed bind size: 18KD
Click image to see more details
Anti-cardiac Troponin C antibody, PA1779, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Protein Target Info & Infographic
Gene/Protein Information For TNNC1 (Source: Uniprot.org, NCBI)
Gene Name
TNNC1
Full Name
Troponin C, slow skeletal and cardiac muscles
Weight
18403 MW
Superfamily
troponin C family
Alternative Names
cardiac troponin C; CMD1Z; CMH13; slow twitch skeletal/cardiac muscle troponin C; TNC; TN-C; TNNC; troponin C type 1 (slow); troponin C, slow skeletal and cardiac muscles; troponin C, slow; troponin C1, slow TNNC1 CMD1Z, CMH13, TN-C, TNC, TNNC troponin C1, slow skeletal and cardiac type troponin C, slow skeletal and cardiac muscles|cardiac troponin C|slow twitch skeletal/cardiac muscle troponin C|troponin C type 1 (slow)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNNC1, check out the TNNC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNNC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-cardiac Troponin C/TNNC1 Antibody (PA1779)
Hello CJ!
PA1779 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li C,Rong X,Qin J,Wang S.An improved two-step method for extraction and purification of primary cardiomyocytes from neonatal mice.J Pharmacol Toxicol Methods.2020 Jul-Aug;104:106887.doi:10.1016/j.vascn.2020.106887.Epub 2020 Jun 11.PMID:32535058.
Species: Mouse
PA1779 usage in article: APP:IF, SAMPLE:CARDIOMYOCYTES, DILUTION:1:100
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-cardiac Troponin C/TNNC1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-cardiac Troponin C/TNNC1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-cardiac Troponin C/TNNC1 Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart muscle using anti-cardiac Troponin C/TNNC1 antibody PA1779. Let me know if you need anything else.
E. Krishna
Verified customer
Asked: 2019-09-18
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-18
Question
I have attached the WB image, lot number and protocol we used for heart muscle using anti-cardiac Troponin C/TNNC1 antibody PA1779. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-20
Question
Our lab want to know about to test anti-cardiac Troponin C/TNNC1 antibody PA1779 on rat heart muscle for research purposes, then I may be interested in using anti-cardiac Troponin C/TNNC1 antibody PA1779 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
The products we sell, including anti-cardiac Troponin C/TNNC1 antibody PA1779, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-05
Question
Will anti-cardiac Troponin C/TNNC1 antibody PA1779 work on goat WB with triceps brachii?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
Our lab technicians have not tested anti-cardiac Troponin C/TNNC1 antibody PA1779 on goat. You can run a BLAST between goat and the immunogen sequence of anti-cardiac Troponin C/TNNC1 antibody PA1779 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat triceps brachii in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
We are currently using anti-cardiac Troponin C/TNNC1 antibody PA1779 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
T. Brown
Verified customer
Asked: 2015-05-20
Answer
The anti-cardiac Troponin C/TNNC1 antibody (PA1779) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-20
Question
I was wanting to use your anti-cardiac Troponin C/TNNC1 antibody for WB for rat heart muscle on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat heart muscle identification?
V. Wu
Verified customer
Asked: 2013-07-18
Answer
It shows on the product datasheet, PA1779 anti-cardiac Troponin C/TNNC1 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat heart muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-18
