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Product Info Summary
| SKU: | EK1512 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human R-Spondin-3 ELISA Kit PicoKine®
View all R-Spondin 3 ELISA kits
SKU/Catalog Number
EK1512
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human R-Spondin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Human RSPO3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human R-Spondin-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1512)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: Q22-H272
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1512 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1512 is reactive to RSPO3 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1512 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of R-Spondin 3
R-spondin-3 is a protein that in humans is encoded by the RSPO3 gene. This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1512-CAP | Anti-Human RSPO3 Pre-coated 96-well strip microplate | 1 |
| EK1512-ST | Human RSPO3 Standard | 2 vials, 10 ng/tube |
| EK1512-DA | Human RSPO3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.042 | 0.065 | 0.099 | 0.145 | 0.263 | 0.522 | 1.135 | 1.906 |
Data Example Images
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Human R-Spondin-3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect R-Spondin 3, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human R-Spondin-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 34 | 151 | 397 | 31 | 138 | 371 |
| Standard deviation | 1.49 | 6.04 | 27.79 | 1.55 | 8.14 | 25.9%4 |
| CV (%) | 4.4% | 4% | 7% | 5% | 5.9% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of R-Spondin 3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 34 | 29 | 32 | 32 | 2.04 | 6.3% |
| Sample 2 | 151 | 180 | 171 | 161 | 165 | 10.84 | 6.5% |
| Sample 3 | 397 | 452 | 458 | 408 | 428 | 26.62 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For RSPO3 (Source: Uniprot.Org, NCBI)
Gene Name
RSPO3
Full Name
R-spondin-3
Weight
30929 MW
Superfamily
R-spondin family
Alternative Names
R-spondin-3;Protein with TSP type-1 repeat;hPWTSR;Roof plate-specific spondin-3;hRspo3;Thrombospondin type-1 domain-containing protein 2;RSPO3;PWTSR, THSD2; Rspo3|2810459H04Rik, AW742308, Cri, Cristin1, Thsd, Thsd2|R-spondin 3|R-spondin-3|R-spondin 3 homolog|cabriolet|cristin 1|cysteine-rich and single thrombospondin domain-containing protein 1|nucleopondin|roof plate-specific spondin-3|thrombospondin, type I, domain 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on RSPO3, check out the RSPO3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RSPO3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human R-Spondin-3 ELISA Kit PicoKine® (EK1512)
Hello CJ!
EK1512 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wnt signaling pathway in early- and late-onset preeclampsia: evaluation with Dickkopf-1 and R-Spondin-3 glycoproteins
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Human R-Spondin-3 ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
G. Anderson
Verified customer
Asked: 2020-08-01
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-08-01
Question
What is the average expiry date of the EK1512 kit?
Verified customer
Asked: 2019-11-18
Answer
The average expiry date of the Human R-Spondin-3 ELISA Kit PicoKine EK1512 and other ELISA kits is within 5-6 months at 4°C and 10-12 months at -20°C from when the customer receives the product.
Boster Scientific Support
Answered: 2019-11-18
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of R-Spondin 3 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the R-Spondin 3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-10-11
Question
Q: Can R-Spondin 3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
E. Young
Verified customer
Asked: 2019-03-11
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-03-11
Question
Q: if the enzyme conjugated R-Spondin 3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the R-Spondin 3 antigen?
Verified Customer
Verified customer
Asked: 2014-10-31
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-10-31
Question
Q: can I use citrate plasma as samples in Human R-Spondin 3 Picokine® ELISA Kit (Catalog # EK1512)?
Verified Customer
Verified customer
Asked: 2014-05-06
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of R-Spondin 3 causing disruption of its protein structure. R-Spondin 3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the R-Spondin 3 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2014-05-06
