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Product Info Summary
| SKU: | A06284-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SEC22B Antibody Picoband®
SKU/Catalog Number
A06284-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SEC22B Antibody Picoband® catalog # A06284-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SEC22B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06284-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SEC22B recombinant protein (Position: M6-A197).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06284-2 is reactive to SEC22B in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
24 kDa
Calculated molecular weight
49655 MW
Background of SEC22B
Vesicle-trafficking protein SEC22b is a protein that in humans is encoded by the SEC22B gene. The protein encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06284-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human U-87 MG whole cell, human Hela whole cell
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SEC22B using anti-SEC22B antibody (A06284-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87 MG whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC22B antigen affinity purified polyclonal antibody (Catalog # A06284-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC22B at approximately 24 kDa. The expected band size for SEC22B is at 25 kDa.
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Figure 2. Flow Cytometry analysis of K562 cells using anti-SEC22B antibody (A06284-2).
Overlay histogram showing K562 cells stained with A06284-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC22B Antibody (A06284-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SEC22B (Source: Uniprot.org, NCBI)
Gene Name
SEC22B
Full Name
Vesicle-trafficking protein SEC22b
Weight
49655 MW
Superfamily
synaptobrevin family
Alternative Names
ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL2; HUB SEC22B ERS-24, SEC22L1 SEC22 homolog B, vesicle trafficking protein vesicle-trafficking protein SEC22b|ER-Golgi SNARE of 24 kDa|SEC22 vesicle trafficking protein homolog B|SEC22 vesicle trafficking protein-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEC22B, check out the SEC22B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEC22B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SEC22B Antibody Picoband® (A06284-2)
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