Anti-SCIMP Purified Monoclonal Antibody

Product Name

Anti-SCIMP Purified Monoclonal Antibody

View all Scimp Antibodies

SKU/Catalog Number

M12849

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SCIMP Purified Monoclonal Antibody (Catalog# M12849). Tested in Flow Cytometry, IP, WB, ICC application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-SCIMP Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12849)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

NVL-07

Isotype

Mouse IgG2a

Immunogen

Recombinant intracellular part of human SCIMP. The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.

Reactive Species

M12849 is reactive to SCIMP in Human

Reconstitution

Observed Molecular Weight

42 kDa

Calculated molecular weight

16618 MW

Background of Scimp

SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M12849 is guaranteed for Flow Cytometry, IP, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: Intracellular staining.
Western blotting: 1-2 μg/ml.

Validation Images & Assay Conditions

Gene/Protein Information For SCIMP (Source: Uniprot.org, NCBI)

Alternative Names

NVL

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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