Product Info Summary
SKU: | A01254-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SCF KITLG Antibody
View all SCF/c-kit Ligand Antibodies
SKU/Catalog Number
A01254-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SCF KITLG Antibody catalog # A01254-2. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCF KITLG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01254-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MDC1 around the phosphorylation site of S513.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01254-2 is reactive to KITLG in Human
Applications
A01254-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
30899 MW
Background of SCF/c-kit Ligand
Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress By similarity.
Delia J. Hern¨¢ndez-Deviez, Hum. Mol. Genet., Jan 2006; 15: 129 - 142.
Yanchao Huang, FASEB J, Mar 2007; 21: 732 - 742.
Mengfatt Ho, Hum. Mol. Genet., Sep 2004; 13: 1999 - 2010.
Eriko Fujita, Hum. Mol. Genet., Mar 2007; 16: 618 - 629.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:100-1:500
FC: 1:50-1:100
Validation Images & Assay Conditions
![a01254 2 anti scf kitlg antibody icc testing 2 a01254 2 anti scf kitlg antibody icc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01254-2-anti-scf-kitlg-antibody-icc-testing-2.jpg)
Click image to see more details
Figure 2. Immunocytochemistry staining of KITLG using Anti-SCF KITLG Antibody (A01254-2).
ICC staining SCF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde![a01254 2 anti scf kitlg antibody wb testing 1 a01254 2 anti scf kitlg antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01254-2-anti-scf-kitlg-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-SCF KITLG Antibody A01254-2
Western blot analysis of SCF on Raji cells lysates using anti-SCF antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For KITLG (Source: Uniprot.org, NCBI)
Gene Name
KITLG
Full Name
Kit ligand
Weight
30899 MW
Superfamily
SCF family
Alternative Names
c-kit Ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SHEP7; SLF; steel factor KITLG DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SCF, SF, SHEP7, SLF KIT ligand kit ligand|c-Kit ligand|familial progressive hyperpigmentation 2|mast cell growth factor|steel factor|stem cell factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KITLG, check out the KITLG Infographic
![KITLG infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KITLG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCF KITLG Antibody (A01254-2)
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