Human Estrodial (E2) ELISA Kit (Competitive ELISA)

Product Name

Human Estrodial (E2) ELISA Kit (Competitive ELISA)

See all 17 beta-HSD1/HSD17B1 products

SKU/Catalog Number

EK7049

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Estrodial (E2). 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Estrodial (E2) ELISA Kit (Competitive ELISA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7049)

Clonality of Antibodies

See Datasheet for details

Sensitivity

40 pg/ml

Assay Range

50-1200 pg/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7049 is reactive to HSD17B1 in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7049 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity	Volume	Buffers
Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Goat Anti- Rabbit Secondary Antibody, Polystyrene micro-well plate
Human Estrodial (E2) Standards (S0~S5)	6	0.5ml	Estrodial (E2) (0, 50, 120, 250, 500, 1200 pg/ml), 0.02M PBS, 50% detoxification serum, 0.1% Proclin-300
HRP Conjugated antigen	1	6ml	HRP Conjugated Human Estrodial (E2), 0.02M PBS, 2% BSA, 0.01% azophloxine, 0.1% Proclin-300
Antibody	1	6ml	Rabbit anti- Human Estrodial (E2) Antibody, 0.02M PBS, 2% BSA, 0.1% Proclin-300, 0.01% Evans Blue
Controls	2	0.5ml	Estrodial (E2), 100% natural protein, 0.1% Proclin-300 Range of Control 1: 84-156 pg/mL Range of Control 2: 350-650 pg/mL
20X Wash Buffer Concentrate	1	15ml	0.2M PBS containing 0.5% tween 20
Color Developing Reagent A	1	7ml	11m mol/L Urea hydrogen peroxide
Color Developing Reagent B	1	7ml	2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution	1	7ml	2mol/L Sulphuric acid
Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

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