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Product Info Summary
| SKU: | EK0509 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA). |
Product info
Product Name
Mouse SCF/KITLG/Kit ligand ELISA Kit PicoKine®
View all SCF/c-kit Ligand ELISA kits
SKU/Catalog Number
EK0509
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SCF ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Kitlg in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SCF/KITLG/Kit ligand ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0509)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: K26-A190
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0509 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0509 is reactive to Kitlg in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
Application Guarantee
EK0509 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SCF/c-kit Ligand
Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0509-CAP | Anti-Mouse Kitlg Pre-coated 96-well strip microplate | 1 |
| EK0509-ST | Mouse Kitlg Standard | 2 vials, 10 ng/tube |
| EK0509-DA | Mouse Kitlg Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.25 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.055 | 0.098 | 0.124 | 0.215 | 0.399 | 0.768 | 1.468 | 2.036 |
Data Example Images
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Mouse SCF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SCF/c-kit Ligand, Dilution ratio of 1:1, concentration in serum and plasma is around 100 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SCF/KITLG/Kit ligand ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 75 | 190 | 820 | 81 | 186 | 861 |
| Standard deviation | 7%5 | 10.26 | 42.64 | 6.96 | 12.46 | 45.4%3 |
| CV (%) | 7% | 5.4% | 5.2% | 8.6% | 6.7% | 5.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SCF/c-kit Ligand in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 75 | 88 | 85 | 89 | 84 | 5.53 | 6.5% |
| Sample 2 | 190 | 214 | 186 | 211 | 200 | 12.37 | 6.1% |
| Sample 3 | 820 | 795 | 798 | 947 | 840 | 62.52 | 7.4% |
Protein Target Info & Infographic
Gene/Protein Information For Kitlg (Source: Uniprot.Org, NCBI)
Gene Name
Kitlg
Full Name
Kit ligand
Weight
30645 MW
Superfamily
SCF family
Alternative Names
Kit ligand;Hematopoietic growth factor KL;Mast cell growth factor;MGF;Steel factor;Stem cell factor;SCF;c-Kit ligand;Soluble KIT ligand;sKITLG;Kitlg;Kitl, Mgf, Sl, Slf; KITLG DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SCF, SF, SHEP7, SLF KIT ligand kit ligand|c-Kit ligand|familial progressive hyperpigmentation 2|mast cell growth factor|steel factor|stem cell factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Kitlg, check out the Kitlg Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Kitlg: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse SCF/KITLG/Kit ligand ELISA Kit PicoKine® (EK0509)
Hello CJ!
EK0509 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
GM-CSF Inhibits c-Kit and SCF Expression by Bone Marrow-Derived Dendritic Cells
Ilomastat contributes to the survival of mouse after irradiation via promoting the recovery of hematopoietic system
GM-CSF Inhibits c-Kit and SCF Expression by Bone Marrow-Derived Dendritic Cells
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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10 Customer Q&As for Mouse SCF/KITLG/Kit ligand ELISA Kit PicoKine®
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of SCF in Mouse plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SCF ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-08-02
Question
Q: how to proceed with the analysis of ELISA data? I measured SCF level in plasma.
Verified Customer
Verified customer
Asked: 2019-04-06
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-04-06
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-11-03
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-11-03
Question
Q: Can SCF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-10-03
Question
Q: is it okay to use heparin plasma as samples in Mouse SCF Picokine® ELISA Kit (Catalog # EK0509)?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of SCF causing degradation of its protein structure. SCF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the SCF ELISA, treating samples with various anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-05-03
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification SCF using the EK0509 Mouse SCF Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-28
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-04-28
Question
Q: What is the optimal O.D. value for SCF ELISA kit? I used your SCF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SCF even though the O.D. values are not very high?
W. Smith
Verified customer
Asked: 2018-04-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SCF ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SCF that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-04-18
Question
Q: if the enzyme conjugated SCF antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SCF antigen?
Y. Zhao
Verified customer
Asked: 2018-03-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-03-28
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-03-27
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-03-27
Question
Q: how can I thaw whole blood sample for SCF ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-11-27
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SCF for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-11-27
