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Product Info Summary
| SKU: | A06272-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SCC112/PDS5A Antibody Picoband®
SKU/Catalog Number
A06272-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SCC112/PDS5A Antibody Picoband® catalog # A06272-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCC112/PDS5A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06272-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PDS5A recombinant protein (Position: M1-R1337).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06272-1 is reactive to PDS5A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
151 kDa
Calculated molecular weight
49655 MW
Background of SCC112
Sister chromatid cohesion protein PDS5 homolog A is a protein that in humans is encoded by the PDS5A gene. The protein encoded by this gene binds to the cohesin complex and associates with chromatin through most of the cell cycle. The encoded protein may play a role in regulating sister chromatid cohesion during mitosis. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06272-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell, human HEK293 whole cell, rat stomach tissue, rat pancrease tissue, mouse stomach tissue, mouse pancrease tissue
ICC/IF: U20S cell
FCM: A431 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SCC112/PDS5A using anti-SCC112/PDS5A antibody (A06272-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: rat stomach tissue lysates,
Lane 4: rat pancrease tissue lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse pancrease tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCC112/PDS5A antigen affinity purified polyclonal antibody (Catalog # A06272-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCC112/PDS5A at approximately 151 kDa. The expected band size for SCC112/PDS5A is at 151 kDa.
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Figure 2. IF analysis of SCC112/PDS5A using anti-SCC112/PDS5A antibody (A06272-1).
SCC112/PDS5A was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SCC112/PDS5A Antibody (A06272-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of A431 cells using anti-SCC112/PDS5A antibody (A06272-1).
Overlay histogram showing A431 cells stained with A06272-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SCC112/PDS5A Antibody (A06272-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PDS5A (Source: Uniprot.org, NCBI)
Gene Name
PDS5A
Full Name
Sister chromatid cohesion protein PDS5 homolog A
Weight
49655 MW
Superfamily
PDS5 family
Alternative Names
ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL2; HUB PDS5A PIG54, SCC-112, SCC112 PDS5 cohesin associated factor A sister chromatid cohesion protein PDS5 homolog A|PDS5, regulator of cohesion maintenance, homolog A|cell proliferation-inducing gene 54 protein|sister chromatid cohesion protein 112
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PDS5A, check out the PDS5A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PDS5A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCC112/PDS5A Antibody Picoband® (A06272-1)
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