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Product Info Summary
| SKU: | A07224-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-SAR1A Antibody (Center)
SKU/Catalog Number
A07224-1
Size
80 µl
Description
Boster Bio Anti-SAR1A Antibody (Center) (Catalog # A07224-1). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SAR1A Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07224-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This SAR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-149 amino acids from the Central region of human SAR1A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07224-1 is reactive to SAR1A in Human
Reconstitution
Calculated molecular weight
22367 Da
Background of SAR1
SAR1A is involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity) and required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07224-1 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from 293,HT1080 cell line,human placenta,mouse liver and lung tissue (from left to right),using SAR1A Antibody (Center). A07224-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Formalin-fixed and paraffin-embedded human lung carcinoma with SAR1A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of NCI-H292 cells using SAR1A Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For SAR1A (Source: Uniprot.org, NCBI)
Gene Name
SAR1A
Full Name
GTP-binding protein SAR1a
Weight
22367 Da
Superfamily
small GTPase superfamily
Alternative Names
GTP-binding protein SAR1a, COPII-associated small GTPase, SAR1A, SAR1, SARA, SARA1 Sar1a|1600019H17Rik, S, Sara, Sara1|secretion associated Ras related GTPase 1A|GTP-binding protein SAR1a|SAR1 gene homolog A|SAR1a gene homolog 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SAR1A, check out the SAR1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SAR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SAR1A Antibody (Center) (A07224-1)
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