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Product Info Summary
| SKU: | M07225-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RYK Rabbit Monoclonal Antibody
SKU/Catalog Number
M07225-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-RYK Rabbit Monoclonal Antibody catalog # M07225-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RYK Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07225-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
24R41
Isotype
IgG
Immunogen
A synthesized peptide derived from human RYK
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M07225-1 is reactive to RYK in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
72 kDa
Calculated molecular weight
130159 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07225-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human MCF-7 whole cell, human A431 whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RYK using anti-RYK antibody (M07225-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RYK antigen affinity purified monoclonal antibody (Catalog # M07225-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RYK at approximately 72 kDa. The expected band size for RYK is at 68 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RYK (Source: Uniprot.org, NCBI)
Gene Name
RYK
Full Name
Tyrosine-protein kinase RYK
Weight
130159 MW
Superfamily
protein kinase superfamily
Alternative Names
Integrin alpha-E;HML-1 antigen;Integrin alpha-IEL;Mucosal lymphocyte 1 antigen;CD103;Integrin alpha-E light chain;Integrin alpha-E heavy chain;ITGAE; RYK D3S3195, JTK5, JTK5A1, RYK receptor like tyrosine kinase tyrosine-protein kinase RYK|JTK5A protein tyrosine kinase|hydroxyaryl-protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RYK, check out the RYK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RYK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RYK Rabbit Monoclonal Antibody (M07225-1)
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