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Product Info Summary
| SKU: | M00493 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-RUNX3 Mouse Monoclonal Antibody [Clone ID: OTI2G1]
View all RUNX3/CBFA3 Antibodies
SKU/Catalog Number
M00493
Size
100 µl
Description
Boster Bio RUNX3 mouse monoclonal antibody,clone OTI2G1. Catalog# M00493. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RUNX3 Mouse Monoclonal Antibody [Clone ID: OTI2G1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00493)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI2G1
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human RUNX3 (NP_001026850) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00493 is reactive to RUNX3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
44.356kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00493 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RUNX3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RUNX3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RUNX3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUNX3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUNX3 (1:2000).
Protein Target Info & Infographic
Gene/Protein Information For RUNX3 (Source: Uniprot.org, NCBI)
Gene Name
RUNX3
Full Name
Runt-related transcription factor 3
Weight
44.356kDa
Alternative Names
Acute myeloid leukemia 2 protein; AML2; AML2SL3/AKV core-binding factor alpha C subunit; CBFA3; CBFA3MGC16070; CBF-alpha-3; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2A3; PEBP2A3FLJ34510; PEBP2AC; PEBP2-alpha C; runt domain, alpha subunit 3; runt-related transcription factor 3; RUNX3; SL3-3 enhancer factor 1 alpha C subunit; transcription factor AML2 RUNX3 AML2, CBFA3, PEBP2aC RUNX family transcription factor 3 runt-related transcription factor 3|CBF-alpha-3|PEA2 alpha C|PEBP2 alpha C|SL3-3 enhancer factor 1 alpha C subunit|SL3/AKV core-binding factor alpha C subunit|acute myeloid leukemia 2 protein|acute myeloid leukemia gene 2|core-binding factor subunit alpha-3|core-binding factor, runt domain, alpha subunit 3|oncogene AML-2|polyomavirus enhancer-binding protein 2 alpha C subunit|runt related transcription factor 3|transcription factor AML2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RUNX3, check out the RUNX3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RUNX3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RUNX3 Mouse Monoclonal Antibody [Clone ID: OTI2G1] (M00493)
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Customer Q&As
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15 Customer Q&As for Anti-RUNX3 Mouse Monoclonal Antibody [Clone ID: OTI2G1]
Question
Will anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493 work for IHC with cervix carcinoma?
J. Carter
Verified customer
Asked: 2020-05-08
Answer
According to the expression profile of cervix carcinoma, RUNX3 is highly expressed in cervix carcinoma. So, it is likely that anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2020-05-08
Question
We have observed staining in human skin. Any tips? Is anti-RUNX3 (human) Monoclonal antibody (R3-6E9) supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
Based on literature skin does express RUNX3. Based on Uniprot.org, RUNX3 is expressed in tonsil, skin, cervix carcinoma, among other tissues. Regarding which tissues have RUNX3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-17
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-13
Question
Our lab want to know about to test anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493 on human cervix carcinoma for research purposes, then I may be interested in using anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The products we sell, including anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-31
Question
My colleagues were content with the WB result of your anti-RUNX3 (human) Monoclonal antibody (R3-6E9) . However we have been able to see positive staining in tonsil nucleus using this antibody. Is that expected? Could you tell me where is RUNX3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
From what I have seen in literature, tonsil does express RUNX3. Generally RUNX3 expresses in nucleus. Regarding which tissues have RUNX3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-25
Question
We are currently using anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
The anti-RUNX3 (human) Monoclonal antibody (R3-6E9) (M00493) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-21
Question
I was wanting to use your anti-RUNX3 (human) Monoclonal antibody (R3-6E9) for IHC for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
As indicated on the product datasheet, M00493 anti-RUNX3 (human) Monoclonal antibody (R3-6E9) has been validated for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-11
Question
Is this M00493 anti-RUNX3 (human) Monoclonal antibody (R3-6E9) reactive to the isotypes of RUNX3?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
The immunogen of M00493 anti-RUNX3 (human) Monoclonal antibody (R3-6E9) is Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-06
Question
Is there a BSA free version of anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493 available?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-20
Question
Would M00493 anti-RUNX3 (human) Monoclonal antibody (R3-6E9) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
It shows on the product datasheet, M00493 anti-RUNX3 (human) Monoclonal antibody (R3-6E9) as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-19
Question
Can you help my question with product M00493, anti-RUNX3 (human) Monoclonal antibody (R3-6E9) . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00493 anti-RUNX3 (human) Monoclonal antibody (R3-6E9) , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-16
Question
Our lab used your anti-RUNX3 (human) Monoclonal antibody (R3-6E9) for IHC on cervix carcinoma in the past. I am using human, and We are going to use the antibody for IP next. I was wanting to use examining cervix carcinoma as well as skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
I viewed the website and datasheets of our anti-RUNX3 (human) Monoclonal antibody (R3-6E9) and it appears that M00493 has been validated on human in both IHC and IP. Thus M00493 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-03-22
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493. Please let me know if you require anything else.
R. Lewis
Verified customer
Asked: 2018-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-21
Question
I see that the anti-RUNX3 (human) Monoclonal antibody (R3-6E9) M00493 works with IHC, what is the protocol used to produce the result images on the product page?
Z. Parker
Verified customer
Asked: 2014-05-22
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-05-22
Question
Is a blocking peptide available for product anti-RUNX3 (human) Monoclonal antibody (R3-6E9) (M00493)?
T. Carter
Verified customer
Asked: 2014-01-24
Answer
We do provide the blocking peptide for product anti-RUNX3 (human) Monoclonal antibody (R3-6E9) (M00493). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-01-24
