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Product Info Summary
| SKU: | M00442 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RUNX2 Monoclonal Antibody
View all RUNX2/CBFA1 Antibodies
SKU/Catalog Number
M00442
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RUNX2 Monoclonal Antibody catalog # M00442. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RUNX2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00442)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HCO-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RUNX2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00442 is reactive to RUNX2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
57 kDa
Calculated molecular weight
81373 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00442 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
Positive Control
WB: human THP-1 whole cell, human U251 whole cell, human RT4 whole cell
IHC: human colon tissue
ICC/IF: Saos-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RUNX2 using anti-RUNX2 antibody (M00442).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified monoclonal antibody (Catalog # M00442) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 57 kDa. The expected band size for RUNX2 is at 57 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon, using RUNX2 Antibody.
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Immunofluorescent analysis of Saos-2 cells, using RUNX2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For RUNX2 (Source: Uniprot.org, NCBI)
Gene Name
RUNX2
Full Name
Runt-related transcription factor 2
Weight
81373 MW
Alternative Names
Death domain-associated protein 6;Daxx;hDaxx;ETS1-associated protein 1;EAP1;Fas death domain-associated protein;DAXX;BING2, DAP6; RUNX2 AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA RUNX family transcription factor 2 runt-related transcription factor 2|PEA2-alpha A|PEBP2-alpha A|SL3-3 enhancer factor 1 alpha A subunit|SL3/AKV core-binding factor alpha A subunit|acute myeloid leukemia 3 protein|core-binding factor, runt domain, alpha subunit 1|oncogene AML-3|osteoblast-specific transcription factor 2|polyomavirus enhancer-binding protein 2 alpha A subunit|runt related transcription factor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RUNX2, check out the RUNX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RUNX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RUNX2 Monoclonal Antibody (M00442)
Hello CJ!
M00442 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen G,Wang Q,Li Z,Yang Q,Liu Y,Du Z,Zhang G,Song Y.Circular RNA CDR1as promotes adipogenic and suppresses osteogenic differentiation of BMSCs in steroid-induced osteonecrosis of the femoral head.Bone.2020 Apr;133:115258.doi:10.1016/j.bone.2020.115258.Epu
Species: Human
M00442 usage in article: APP:WB, SAMPLE:BMSCS, DILUTION:NA
Effects of Intermittent Administration of Parathyroid Hormone (1-34) on Bone Differentiation in Stromal Precursor Antigen-1 Positive Human Periodontal Ligament Stem Cells
Yao S, Zhao W, Ou Q, Liang L, Lin X, Wang Y. Stem Cells Int. 2017;2017:3028647. doi: 10.1155/2017/3028647. Epub 2017 Oct 29. MicroRNA-214 Suppresses Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting ATF4
Liu Q, Ma Y, Wang J, Zhu X, Yang Y, Mei Y. PLoS One. 2017 Mar 2;12(3):e0172693. doi: 10.1371/journal.pone.0172693. eCollection 2017. Demineralized bone matrix used for direct pulp capping in rats
Liu F, Zhong H, Liang Jy, Fu P, Luo Zj, Zhou L, Gou R, Huang J. J Zhejiang Univ Sci B. 2010 Dec;11(12):905-11. Doi: 10.1631/Jzus.B1000119. Effect Of High Glucose Levels On The Calcification Of Vascular Smooth Muscle Cells By Inducing Osteoblastic ...
Mu C, Lv T, Wang Z, Ma S, Ma J, Liu J, Yu J, Mu J. Biomed Res Int. 2014;2014:494378. Doi: 10.1155/2014/494378. Epub 2014 Apr 15. Mechanical Stress Stimulates The Osteo/Odontoblastic Differentiation Of Human Stem Cells From Apical Papilla Via Erk 1...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-RUNX2 Monoclonal Antibody
Question
I see that the anti-RUNX2 Monoclonal antibody M00442 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-30
Question
Is this M00442 anti-RUNX2 Monoclonal antibody reactive to the isotypes of RUNX2?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The immunogen of M00442 anti-RUNX2 Monoclonal antibody is A synthesized peptide derived from human RUNX2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-31
Question
Will M00442 anti-RUNX2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
It shows on the product datasheet, M00442 anti-RUNX2 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-22
Question
Is there a BSA free version of anti-RUNX2 Monoclonal antibody M00442 available?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RUNX2 Monoclonal antibody M00442 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibia using anti-RUNX2 Monoclonal antibody M00442. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-28
Question
We have seen staining in mouse osteoblast. Do you have any suggestions? Is anti-RUNX2 Monoclonal antibody supposed to stain osteoblast positively?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
From what I have seen in literature osteoblast does express RUNX2. From what I have seen in Uniprot.org, RUNX2 is expressed in tibia, osteoblast, cervix carcinoma, among other tissues. Regarding which tissues have RUNX2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336
Osteoblast, Pubmed ID: 12145306
Boster Scientific Support
Answered: 2019-08-13
Question
Is a blocking peptide available for product anti-RUNX2 Monoclonal antibody (M00442)?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
We do provide the blocking peptide for product anti-RUNX2 Monoclonal antibody (M00442). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-26
Question
I was wanting to use your anti-RUNX2 Monoclonal antibody for IHC for human tibia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tibia identification?
S. Jackson
Verified customer
Asked: 2019-03-28
Answer
As indicated on the product datasheet, M00442 anti-RUNX2 Monoclonal antibody has been tested for IF, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human tibia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-28
Question
We bought anti-RUNX2 Monoclonal antibody for IF on osteoblast a few years ago. I am using mouse, and We want to use the antibody for IHC next. Our lab want to know about examining osteoblast as well as tibia in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
I viewed the website and datasheets of our anti-RUNX2 Monoclonal antibody and it seems that M00442 has been tested on mouse in both IF and IHC. Thus M00442 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-05
Question
Can you help my question with product M00442, anti-RUNX2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00442 anti-RUNX2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-18
Question
We are currently using anti-RUNX2 Monoclonal antibody M00442 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
The anti-RUNX2 Monoclonal antibody (M00442) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-11
Question
My question regards to test anti-RUNX2 Monoclonal antibody M00442 on human tibia for research purposes, then I may be interested in using anti-RUNX2 Monoclonal antibody M00442 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-29
Answer
The products we sell, including anti-RUNX2 Monoclonal antibody M00442, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-29
Question
Does anti-RUNX2 Monoclonal antibody M00442 work for IHC with tibia?
T. Zhao
Verified customer
Asked: 2015-06-16
Answer
According to the expression profile of tibia, RUNX2 is highly expressed in tibia. So, it is likely that anti-RUNX2 Monoclonal antibody M00442 will work for IHC with tibia.
Boster Scientific Support
Answered: 2015-06-16
Question
Our team were content with the WB result of your anti-RUNX2 Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleus using this antibody. Is that expected? Could you tell me where is RUNX2 supposed to be expressed?
G. Yang
Verified customer
Asked: 2014-12-30
Answer
Based on literature, cervix carcinoma does express RUNX2. Generally RUNX2 expresses in nucleus. Regarding which tissues have RUNX2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336
Osteoblast, Pubmed ID: 12145306
Boster Scientific Support
Answered: 2014-12-30
Question
See below the WB image, lot number and protocol we used for tibia using anti-RUNX2 Monoclonal antibody M00442. Please let me know if you require anything else.
H. Johnson
Verified customer
Asked: 2013-06-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-07
