Product Info Summary
SKU: | EK0462 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Mouse |
Application: | ELISA |
Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0462
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0462)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Y18-C477
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0462 for more details
Cross-reactivity
There is cross-reactivity with MMP-10 approximately 2% and no detectable cross-reactivity with other MMPs.
Reactive Species
EK0462 is reactive to MMP3 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0462 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-3
Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK0462-CAP | Anti-Mouse MMP3 Pre-coated 96-well strip microplate | 1 |
EK0462-ST | Mouse MMP3 Standard | 2 vials, 10 ng/tube |
EK0462-DA | Mouse MMP3 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.027 | 0.097 | 0.162 | 0.265 | 0.471 | 0.803 | 1.333 | 1.952 |
Data Example Images
Click image to see more details
Mouse MMP-3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-3, Dilution ratio of 1:40, concentration in serum and plasma is around 60 ng/ml..
Some articles we found to cite concentrations of MMP-3 in samples: 24167138 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 1427 | 1427 | 4432 | 300 | 1524 | 4802 |
Standard deviation | 17.14 | 68.49 | 230.46 | 21 | 102.1 | 288.12 |
CV (%) | 6.1% | 4.8% | 5.2% | 7% | 6.7% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-3 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 281 | 283 | 271 | 287 | 280 | 5.89 | 2.1% |
Sample 2 | 1427 | 1569 | 1617 | 1589 | 1550 | 73.31 | 4.7% |
Sample 3 | 4432 | 4951 | 4788 | 4684 | 4713 | 188.46 | 3.9% |
Protein Target Info & Infographic
Gene/Protein Information For MMP3 (Source: Uniprot.Org, NCBI)
Gene Name
MMP3
Full Name
Stromelysin-1
Weight
53.977kDa
Superfamily
peptidase M10A family
Alternative Names
CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1 MMP3 CHDS6, MMP-3, SL-1, STMY, STMY1, STR1 matrix metallopeptidase 3 stromelysin-1|matrix metalloproteinase 3 (stromelysin 1, progelatinase)|proteoglycanase|transin-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MMP3, check out the MMP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine® (EK0462)
Hello CJ!
EK0462 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Learning and memory deficits and alzheimers disease-like changes in mice after chronic exposure to microcystin-LR
miR‐134 inhibits osteosarcoma cell invasion and metastasis through targeting MMP1 and MMP3 in vitro and in vivo
Xie J, Huo S, Li Y, Dai J, Xu H, Yin ZQ. Cell Transplant. 2017 Jun 9;26(6):967-982. doi: 10.3727/096368917X694994. Epub 2017 Feb 9. Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the M
Zhao J, Fan X, Zhang Q, Sun F, Li X, Xiong C, Zhang C, Fan H. J Biomed Mater Res A. 2014 Feb;102(2):373-80. Doi: 10.1002/Jbm.A.34711. Epub 2013 May 14. Chitosan-Plasmid Dna Nanoparticles Encoding Small Hairpin Rna Targeting Mmp-3 And -13 To Inhibi...
??gren Ms, Schnabel R, Christensen Lh, Mirastschijski U. Eur J Cell Biol. 2015 Jan;94(1):12-21. Doi: 10.1016/J.Ejcb.2014.10.001. Epub 2014 Oct 23. Tumor Necrosis Factor-??-Accelerated Degradation Of Type I Collagen In Human Skin Is Associated With...
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
12 Customer Q&As for Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated MMP-3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-3 antigen?
Verified Customer
Verified customer
Asked: 2020-05-26
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-05-26
Question
Is this kit reactive to pro-, active, and TIMP-complexed MMP3 protein?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
EK0462 is reactive to total MMP3 including pro-, active, and TIMP-complexed MMP3 protein.
Boster Scientific Support
Answered: 2019-11-21
Question
Is EK0462 reactive to pro-, active, and TIMP-complexed MMP3 protein? What proteins have been tested for cross-reactivity?
Verified customer
Asked: 2019-04-24
Answer
The Mouse MMP-3 ELISA Kit PicoKine™ (EK0462) is reactive to total MMP3 including pro-, active, and TIMP-complexed MMP3 protein. The proteins listed below have been tested for cross-reactivity. Recombinant mouse MMP-2 protein Recombinant mouse MMP-9 protein Recombinant mouse TIMP-1 protein
Boster Scientific Support
Answered: 2019-04-24
Question
Q: which procedure should I follow in order to thaw whole blood sample for MMP-3 ELISA after freezing?
K. Babu
Verified customer
Asked: 2019-03-01
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-03-01
Question
Q: how do I analyze ELISA data? I have performed MMP-3 level in plasma.
J. Bhasin
Verified customer
Asked: 2018-12-30
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-12-30
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-12-02
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-12-02
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
D. Phillips
Verified customer
Asked: 2018-07-10
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-07-10
Question
I am interested in your EK0462 MMP3 ELISA kit, may I know if this kit react to pro-, active, and TIMP-complexed MMP3 protein? And may I know what proteins you have tested with no cross reactivity concerned?
Verified Customer
Verified customer
Asked: 2018-07-04
Answer
EK0462 is reactive to total MMP3 including pro-, active, and TIMP-complexed MMP3 protein. The proteins listed below have been tested for cross-reactivity. Recombinant mouse MMP-2 protein Recombinant mouse MMP-9 protein Recombinant mouse TIMP-1 protein
Boster Scientific Support
Answered: 2018-07-04
Question
I am interested in your EK0462 MMP3 ELISA kit, may I know have you tested the kit with serum or plasma samples and may I know if the samples concentrations you tested by the kit and if you have suggested dilution ratio to use with this kit?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
The concentration range of MMP3 in serum and plasma samples is 1.5-30ng/ml. Recommended dilution ratio for normal serum and plasma samples is from 1:1 to 1:20.
Boster Scientific Support
Answered: 2018-05-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-07-05
Question
Q: Can MMP-3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Phillips
Verified customer
Asked: 2016-05-28
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2016-05-28
Question
What proteins have been tested for cross-reactivity?
Verified Customer
Verified customer
Asked: 2014-10-06
Answer
The proteins listed below have been tested for cross-reactivity. <br> Recombinant mouse MMP-2 protein <br> Recombinant mouse MMP-9 protein <br> Recombinant mouse TIMP-1 protein
Boster Scientific Support
Answered: 2014-10-06