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Product Info Summary
| SKU: | A08159-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-RRP1B Antibody Picoband®
SKU/Catalog Number
A08159-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RRP1B Antibody Picoband® catalog # A08159-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RRP1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08159-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RRP1B recombinant protein (Position: E20-K727).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08159-1 is reactive to RRP1B in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
95 kDa
Calculated molecular weight
17136 MW
Background of RRP1B
RRP1B (ribosomal RNA processing 1 homolog B), also known as KIAA0179, is a human gene which is located on Chromosome 21. Enables transcription coactivator activity. Involved in several processes, including cellular response to virus; positive regulation by host of viral transcription; and positive regulation of transcription by RNA polymerase II. Located in chromosome; granular component; and nucleoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08159-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell, human HepG2 whole cell, human Hela whole cell
ICC/IF: MCF-7 cell
FCM: SiHa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RRP1B using anti-RRP1B antibody (A08159-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP1B antigen affinity purified polyclonal antibody (Catalog # A08159-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP1B at approximately 95 kDa. The expected band size for RRP1B is at 84 kDa.
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Figure 2. IF analysis of RRP1B and Tubulin beta using anti-RRP1B antibody (A08159-1) and anti-Tubulin beta antibody (M05613-4).
RRP1B and Tubulin beta was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RRP1B Antibody (A08159-1) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of SiHa cells using anti-RRP1B antibody (A08159-1).
Overlay histogram showing SiHa cells stained with A08159-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RRP1B Antibody (A08159-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RRP1B (Source: Uniprot.org, NCBI)
Gene Name
RRP1B
Full Name
Ribosomal RNA processing protein 1 homolog B
Weight
17136 MW
Superfamily
RRP1 family
Alternative Names
Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; ATP-dependent RNA helicase #46; DEAH box protein 15; DHX15; DBP1, DDX15 RRP1B KIAA0179, NNP1L, Nnp1, PPP1R136, RRP1 ribosomal RNA processing 1B ribosomal RNA processing protein 1 homolog B|RRP1-like protein B|protein phosphatase 1, regulatory subunit 136|ribosomal RNA processing 1 homolog B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RRP1B, check out the RRP1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RRP1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RRP1B Antibody Picoband® (A08159-1)
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