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Product Info Summary
| SKU: | A03902-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RREB1 Antibody Picoband®
SKU/Catalog Number
A03902-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RREB1 Antibody Picoband® catalog # A03902-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RREB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03902-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RREB1 recombinant protein (Position: K732-Q1559).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03902-2 is reactive to RREB1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
160 kDa
Calculated molecular weight
181.42kDa
Background of RREB1
Ras-responsive element-binding protein 1 is a protein that in humans is encoded by the RREB1 gene. The protein encoded by this gene is a zinc finger transcription factor that binds to RAS-responsive elements (RREs) of gene promoters. It has been shown that the calcitonin gene promoter contains an RRE and that the encoded protein binds there and increases expression of calcitonin, which may be involved in Ras/Raf-mediated cell differentiation. Multiple transcript variants encoding several different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03902-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human SH-SY5Y whole cell
FCM: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RREB1 using anti-RREB1 antibody (A03902-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RREB1 antigen affinity purified polyclonal antibody (Catalog # A03902-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RREB1 at approximately 160 kDa. The expected band size for RREB1 is at 160-181 kDa.
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Figure 2. Flow Cytometry analysis of Hela cells using anti-RREB1 antibody (A03902-2).
Overlay histogram showing Hela cells stained with A03902-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RREB1 Antibody (A03902-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RREB1 (Source: Uniprot.org, NCBI)
Gene Name
RREB1
Full Name
Ras-responsive element-binding protein 1
Weight
181.42kDa
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
Fructose-bisphosphate aldolase B; Liver-type aldolase; ALDOB; ALDB RREB1 FINB, HNT, LZ321, RREB-1, Zep-1 ras responsive element binding protein 1 ras-responsive element-binding protein 1|DNA-binding protein|finger protein in nuclear bodies|hindsight homolog|raf-responsive zinc finger protein LZ321|zinc finger motif enhancer-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RREB1, check out the RREB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RREB1 Antibody Picoband® (A03902-2)
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