Product Info Summary
SKU: | A03902 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-RREB1 Antibody
SKU/Catalog Number
A03902
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RREB1 Antibody (Catalog# A03902). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RREB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03902)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RREB1. Immunogen sequence location: 560 - 609
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03902 is reactive to RREB1 in Human, Mouse
Applications
A03902 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
184154 MW
Background of RREB1
Integrin alpha 1 (ITGA1) chain associates with the beta 1 (ITGB1) chain to form a heterodimer that functions as a dual laminin/collagen receptor in neural cells and hematopoietic cells. ITGA1 has a 206-amino acid I domain in its N-terminal half, followed by 3 divalent cation-binding sites and a C-terminal transmembrane domain with a short cytoplasmic tail. It also has 28 potential N-glycosylation sites. Human ITGA1 was expressed in a mouse fibroblast cell line as a 180-kD protein. ITGA1 is involved in the early remodeling of osteoarthritic cartilage and plays an essential role in the regulation of mesenchymal stem cell proliferation and cartilage production. It also plays an essential role in the regulation of MSC proliferation and cartilage production.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of RREB1 expression in HEK293 cell lysates (lane 1), RFP-RREB transfected HEK293 cell lysates (lane 2), M460 cell lysates (lane 3) and T1165 cell lysates (lane 4). RREB1 was detected using rabbit anti-RREB1 Protein A purified polyclonal antibody (Catalog # A03902) at 1:1000. Personal Communication, Shuling Zhang, CCR, NCI, Bethesda, MD.
Protein Target Info & Infographic
Gene/Protein Information For RREB1 (Source: Uniprot.org, NCBI)
Gene Name
RREB1
Full Name
Ras-responsive element-binding protein 1
Weight
184154 MW
Superfamily
krueppel C2H2-type zinc-finger protein family
Alternative Names
DNA-binding protein; FINB; Finger protein in nuclear bodies; hindsight homolog; HNT; LZ321; Raf-responsive zinc finger protein LZ321; ras responsive element binding protein 1; ras-responsive element-binding protein 1; RREB-1; Zep-1; zinc finger motif enhancer binding protein 1; Zinc finger motif enhancer-binding protein 1; zinc finger motif-enhancer binding-protein 1 RREB1 FINB, HNT, LZ321, RREB-1, Zep-1 ras responsive element binding protein 1 ras-responsive element-binding protein 1|DNA-binding protein|finger protein in nuclear bodies|hindsight homolog|raf-responsive zinc finger protein LZ321|zinc finger motif enhancer-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RREB1, check out the RREB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RREB1 Antibody (A03902)
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No publications found for A03902
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Customer Q&As
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5 Customer Q&As for Anti-RREB1 Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-RREB1 antibody A03902. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-29
Question
We are currently using anti-RREB1 antibody A03902 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
N. Krishna
Verified customer
Asked: 2018-08-15
Answer
The anti-RREB1 antibody (A03902) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-15
Question
I have a question about product A03902, anti-RREB1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03902 anti-RREB1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-06
Question
We are interested in to test anti-RREB1 antibody A03902 on human liver for research purposes, then I may be interested in using anti-RREB1 antibody A03902 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Anderson
Verified customer
Asked: 2017-04-03
Answer
The products we sell, including anti-RREB1 antibody A03902, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-03
Question
I see that the anti-RREB1 antibody A03902 works with WB, what is the protocol used to produce the result images on the product page?
Z. Mangal
Verified customer
Asked: 2013-09-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-09-20