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Product Info Summary
| SKU: | M09096 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-RPS4X Antibody (C-Term)
SKU/Catalog Number
M09096
Size
50 µl
Description
Boster Bio Anti-RPS4X Antibody (C-Term) (Catalog # M09096). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RPS4X Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09096)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RPS4X antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 209-243 amino acids from the human region of human RPS4X.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M09096 is reactive to RPS4X in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
29598 Da
Background of RPS4X
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09096 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-RPS4X Antibody (C-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: PC-3 whole cell lysate
Lane 3: HL-60 whole cell lysate
Lane 4: HT-29 whole cell lysate
Lane 5: rat lung lysate
Lane 6: mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 30 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with M09096 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M09096, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For RPS4X (Source: Uniprot.org, NCBI)
Gene Name
RPS4X
Full Name
40S ribosomal protein S4, X isoform
Weight
29598 Da
Superfamily
eukaryotic ribosomal protein eS4 family
Alternative Names
40S ribosomal protein S4, X isoform, SCR10, Single copy abundant mRNA protein, RPS4X, CCG2, RPS4, SCAR RPS4X CCG2, DXS306, RPS4, S4, SCAR, SCR10 ribosomal protein S4 X-linked 40S ribosomal protein S4, X isoform|cell cycle gene 2|single copy abundant mRNA protein|single-copy abundant mRNA|small ribosomal subunit protein eS4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS4X, check out the RPS4X Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS4X: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPS4X Antibody (C-Term) (M09096)
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