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Product Info Summary
| SKU: | M06699 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-RPS27 Antibody (N-Term)
View all Ribosomal protein S27 Antibodies
SKU/Catalog Number
M06699
Size
50 µl
Description
Boster Bio Anti-RPS27 Antibody (N-Term) (Catalog # M06699). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RPS27 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06699)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RPS27 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-37 amino acids from human RPS27.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06699 is reactive to RPS27 in Human
Reconstitution
Calculated molecular weight
9461 Da
Background of Ribosomal protein S27
Required for synthesis of pyridoxal-5-phosphate from vitamin B6.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06699 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-RPS27 Antibody (N-Term) at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: THP-1 whole cell lysate
Lane 4: SK-BR-3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 9 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M06699 staining RPS27 in human brain tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing Jurkat cells stained with M06699 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M06699, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For RPS27 (Source: Uniprot.org, NCBI)
Gene Name
RPS27
Full Name
40S ribosomal protein S27
Weight
9461 Da
Superfamily
eukaryotic ribosomal protein eS27 family
Alternative Names
40S ribosomal protein S27, Metallopan-stimulin 1, MPS-1, RPS27, MPS1 RPS27 DBA17, MPS-1, MPS1, S27 ribosomal protein S27 40S ribosomal protein S27|metallopan-stimulin 1|metallopanstimulin 1|small ribosomal subunit protein eS27
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS27, check out the RPS27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPS27 Antibody (N-Term) (M06699)
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