Product Info Summary
SKU: | A02843 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-RPL5/Ribosomal Protein L5 Antibody
SKU/Catalog Number
A02843
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-RPL5/Ribosomal Protein L5 Antibody (Catalog # A02843). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPL5/Ribosomal Protein L5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02843)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RPL5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02843 is reactive to RPL5 in Human, Mouse, Rat
Applications
A02843 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
34363 MW
Background of RPL5
Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
Frigerio J.-M., Biochim. Biophys. Acta 1262:64-68(1995).
Gregory S.G., Nature 441:315-321(2006).
Odintsova T.I., J. Protein Chem. 22:249-258(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells and Jurkat cells, using RPL5 antibody A02843.
Protein Target Info & Infographic
Gene/Protein Information For RPL5 (Source: Uniprot.org, NCBI)
Gene Name
RPL5
Full Name
60S ribosomal protein L5
Weight
34363 MW
Superfamily
universal ribosomal protein uL18 family
Alternative Names
DBA6,60S ribosomal protein L5; MGC117339; ribosomal protein L5 RPL5 L5, MSTP030, PPP1R135, uL18 ribosomal protein L5 60S ribosomal protein L5|large ribosomal subunit protein uL18|protein phosphatase 1, regulatory subunit 135
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL5, check out the RPL5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL5/Ribosomal Protein L5 Antibody (A02843)
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No publications found for A02843
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Customer Q&As
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7 Customer Q&As for Anti-RPL5/Ribosomal Protein L5 Antibody
Question
My question regards to test anti-RPL5/Ribosomal Protein L5 antibody A02843 on human lymphoblast for research purposes, then I may be interested in using anti-RPL5/Ribosomal Protein L5 antibody A02843 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The products we sell, including anti-RPL5/Ribosomal Protein L5 antibody A02843, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-07
Question
I see that the anti-RPL5/Ribosomal Protein L5 antibody A02843 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-16
Question
We are currently using anti-RPL5/Ribosomal Protein L5 antibody A02843 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
The anti-RPL5/Ribosomal Protein L5 antibody (A02843) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-04
Question
I was wanting to use your anti-RPL5/Ribosomal Protein L5 antibody for WB for human lymphoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphoblast identification?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
It shows on the product datasheet, A02843 anti-RPL5/Ribosomal Protein L5 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lymphoblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-27
Question
Is this A02843 anti-RPL5/Ribosomal Protein L5 antibody reactive to the isotypes of RPL5?
K. Li
Verified customer
Asked: 2019-02-18
Answer
The immunogen of A02843 anti-RPL5/Ribosomal Protein L5 antibody is Synthesized peptide derived from internal of human RPL5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-18
Question
Would anti-RPL5/Ribosomal Protein L5 antibody A02843 work on feline WB with liver?
A. Patel
Verified customer
Asked: 2018-09-21
Answer
Our lab technicians have not validated anti-RPL5/Ribosomal Protein L5 antibody A02843 on feline. You can run a BLAST between feline and the immunogen sequence of anti-RPL5/Ribosomal Protein L5 antibody A02843 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-21
Question
Please see the WB image, lot number and protocol we used for lymphoblast using anti-RPL5/Ribosomal Protein L5 antibody A02843. Please let me know if you require anything else.
T. Walker
Verified customer
Asked: 2016-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-06