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Product Info Summary
| SKU: | A09342 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HLAH Antibody
SKU/Catalog Number
A09342
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-HLAH Antibody (Catalog # A09342). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLAH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09342)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human HLAH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09342 is reactive to HLA-H in Human
Reconstitution
Calculated molecular weight
40892 MW
Background of HLA-H
Involved in the presentation of foreign antigens to the immune system.
Malissen M., Proc. Natl. Acad. Sci. U.S.A. 79:893-897(1982).
Zahedi R.P., J. Proteome Res. 7:526-534(2008).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09342 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from LOVO cells, using HLAH antibody A09342.
Protein Target Info & Infographic
Gene/Protein Information For HLA-H (Source: Uniprot.org, NCBI)
Gene Name
HLA-H
Full Name
Putative HLA class I histocompatibility antigen, alpha chain H
Weight
40892 MW
Superfamily
MHC class I family
Alternative Names
Putative HLA class I histocompatibility antigen, alpha chain H;HLA-12.4;HLA-AR;MHC class I antigen H;HLA-H;HLAH; HLA-H HLAHP major histocompatibility complex, class I, H (pseudogene)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-H, check out the HLA-H Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-H: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HLAH Antibody (A09342)
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No publications found for A09342
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-HLAH Antibody
Question
I have a question about product A09342, anti-HLAH antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09342 anti-HLAH antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-06
Question
Does A09342 anti-HLAH antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
It shows on the product datasheet, A09342 anti-HLAH antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-24
Question
I see that the anti-HLAH antibody A09342 works with WB, what is the protocol used to produce the result images on the product page?
E. Mangal
Verified customer
Asked: 2019-05-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-17
Question
Do you have a BSA free version of anti-HLAH antibody A09342 available?
Verified Customer
Verified customer
Asked: 2018-02-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HLAH antibody A09342 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-26
