Click image to see more details
Product Info Summary
| SKU: | A04311 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RPL3/Ribosomal Protein L3 Antibody
SKU/Catalog Number
A04311
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-RPL3/Ribosomal Protein L3 Antibody (Catalog # A04311). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPL3/Ribosomal Protein L3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04311)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RPL3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04311 is reactive to RPL3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
46109 MW
Background of RPL3
The L3 protein is a component of the large subunit of cytoplasmic ribosomes.
Leffers H.; Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
Shichijo S., Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04311 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HT-29 cells, using RPL3 antibody A04311.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using RPL3 antibody A04311.
Protein Target Info & Infographic
Gene/Protein Information For RPL3 (Source: Uniprot.org, NCBI)
Gene Name
RPL3
Full Name
60S ribosomal protein L3
Weight
46109 MW
Superfamily
universal ribosomal protein uL3 family
Alternative Names
60S ribosomal protein L3;HIV-1 TAR RNA-binding protein B;TARBP-B;RPL3;OK/SW-cl.32; RPL3 ASC-1, L3, TARBP-B ribosomal protein L3 60S ribosomal protein L3|HIV-1 TAR RNA-binding protein B|large ribosomal subunit protein uL3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL3, check out the RPL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL3/Ribosomal Protein L3 Antibody (A04311)
Hello CJ!
No publications found for A04311
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RPL3/Ribosomal Protein L3 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-RPL3/Ribosomal Protein L3 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-RPL3/Ribosomal Protein L3 Antibody
Question
We need to test anti-RPL3/Ribosomal Protein L3 antibody A04311 on rat lung for research purposes, then I may be interested in using anti-RPL3/Ribosomal Protein L3 antibody A04311 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
The products we sell, including anti-RPL3/Ribosomal Protein L3 antibody A04311, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-30
Question
Does anti-RPL3/Ribosomal Protein L3 antibody A04311 work for WB with lung?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
According to the expression profile of lung, RPL3 is highly expressed in lung. So, it is likely that anti-RPL3/Ribosomal Protein L3 antibody A04311 will work for WB with lung.
Boster Scientific Support
Answered: 2020-01-17
Question
Is a blocking peptide available for product anti-RPL3/Ribosomal Protein L3 antibody (A04311)?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We do provide the blocking peptide for product anti-RPL3/Ribosomal Protein L3 antibody (A04311). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-11
Question
I was wanting to use your anti-RPL3/Ribosomal Protein L3 antibody for WB for rat lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lung identification?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
It shows on the product datasheet, A04311 anti-RPL3/Ribosomal Protein L3 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-22
Question
Does A04311 anti-RPL3/Ribosomal Protein L3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
It shows on the product datasheet, A04311 anti-RPL3/Ribosomal Protein L3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-30
Question
Do you have a BSA free version of anti-RPL3/Ribosomal Protein L3 antibody A04311 available?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RPL3/Ribosomal Protein L3 antibody A04311 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-23
Question
We are currently using anti-RPL3/Ribosomal Protein L3 antibody A04311 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
The anti-RPL3/Ribosomal Protein L3 antibody (A04311) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-03
Question
My question regarding product A04311, anti-RPL3/Ribosomal Protein L3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04311 anti-RPL3/Ribosomal Protein L3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-02
Question
See attached the WB image, lot number and protocol we used for lung using anti-RPL3/Ribosomal Protein L3 antibody A04311. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-21
Question
Is this A04311 anti-RPL3/Ribosomal Protein L3 antibody reactive to the isotypes of RPL3?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
The immunogen of A04311 anti-RPL3/Ribosomal Protein L3 antibody is Synthesized peptide derived from internal of human RPL3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-RPL3/Ribosomal Protein L3 antibody A04311. Let me know if you need anything else.
D. Roberts
Verified customer
Asked: 2016-11-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-11-04
Question
Does anti-RPL3/Ribosomal Protein L3 antibody A04311 work on canine WB with pns?
A. Parker
Verified customer
Asked: 2015-12-30
Answer
Our lab technicians have not tested anti-RPL3/Ribosomal Protein L3 antibody A04311 on canine. You can run a BLAST between canine and the immunogen sequence of anti-RPL3/Ribosomal Protein L3 antibody A04311 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine pns in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-30
Question
I see that the anti-RPL3/Ribosomal Protein L3 antibody A04311 works with WB, what is the protocol used to produce the result images on the product page?
O. Wu
Verified customer
Asked: 2014-08-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-08-11
