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Product Info Summary
| SKU: | P05442 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-COXIV (Ser58) COX4I1 Antibody
SKU/Catalog Number
P05442
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-COXIV (Ser58) COX4I1 Antibody (Catalog # P05442). Tested in WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-COXIV (Ser58) COX4I1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P05442)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of mouse COXIV, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Mouse.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P05442 is reactive to COX4i1 in Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
19577 MW
Background of COX4I1
COX, also known as cytochrome c oxidase, has 12 subunits that make up the transmembrane mitochondrial protein. Subunit IV has two isoforms; COXIV-1 and COXIV-2 (Huttermann et al., 2001). COXIV-1 is expressed ubiquitously while COXIV-2 is highly expressed in adult lung and low levels in brain and heart (Huttermann et al., 2001). Phosphorylation of amino acid residue Ser-58 of the COXIV-1 protein is a PKA-dependent regulation of COX and plays an important role in metabolism and CREB cycle activation (AcinPerez et al., 2011).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P05442 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western blot of rat mitochondrial lysate showing specific immunolabeling of the ~17 kDa COXIV phosphorylated at Ser58 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For COX4i1 (Source: Uniprot.org, NCBI)
Gene Name
COX4i1
Full Name
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
Weight
19577 MW
Superfamily
cytochrome c oxidase IV family
Alternative Names
AL024441 antibody, COX 4 antibody, COX IV 1 antibody, COX IV antibody, COX IV-1 antibody, Cox4 antibody, COX41_HUMAN antibody, Cox4a antibody, COX4B antibody, COX4I1 antibody, COX4I2 antibody, COX4L2 antibody, Cytochrome c oxidase polypeptide IV antibody, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody, Cytochrome C Oxidase subunit IV antibody, Cytochrome c oxidase subunit IV isoform 1 antibody, Cytochrome c oxidase subunit IV isoform 2 (lung) antibody, Cytochrome c oxydase subunit 4 antibody, MGC105470 antibody, MGC72016 antibody COX4I1 COX IV-1, COX4, COX4-1, COXIV, COXIV-1, MC4DN16 cytochrome c oxidase subunit 4I1 cytochrome c oxidase subunit 4 isoform 1, mitochondrial|cytochrome c oxidase polypeptide IV|cytochrome c oxidase subunit IV
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COX4i1, check out the COX4i1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COX4i1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-COXIV (Ser58) COX4I1 Antibody (P05442)
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No publications found for P05442
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Customer Q&As
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3 Customer Q&As for Anti-Phospho-COXIV (Ser58) COX4I1 Antibody
Question
Is this P05442 anti-Phospho-COXIV (Ser58) antibody reactive to the isotypes of COX4I1?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The immunogen of P05442 anti-Phospho-COXIV (Ser58) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of mouse COXIV protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-26
Question
Would P05442 anti-Phospho-COXIV (Ser58) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Anderson
Verified customer
Asked: 2018-08-08
Answer
You can see on the product datasheet, P05442 anti-Phospho-COXIV (Ser58) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-08
Question
My lab would like to test anti-Phospho-COXIV (Ser58) antibody P05442 on mouse heart muscle for research purposes, then I may be interested in using anti-Phospho-COXIV (Ser58) antibody P05442 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Wu
Verified customer
Asked: 2013-07-19
Answer
The products we sell, including anti-Phospho-COXIV (Ser58) antibody P05442, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-07-19
