Product Info Summary
SKU: | A10247-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-RPH3A Antibody Picoband™
View all Rabphilin 3A Antibodies
SKU/Catalog Number
A10247-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RPH3A Antibody Picoband™ catalog # A10247-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RPH3A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10247-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RPH3A recombinant protein (Position: R78-Q564).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10247-2 is reactive to Rph3a in Human, Mouse, Rat
Applications
A10247-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
85 kDa
Calculated molecular weight
41409 MW
Background of Rabphilin 3A
Rabphilin-3A is a protein that in humans is encoded by the RPH3A gene. The protein encoded by this gene is thought to be an effector for RAB3A, which is a small G protein that acts in the late stages of neurotransmitter exocytosis. The encoded protein may be involved in neurotransmitter release and synaptic vesicle traffic.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RPH3A using anti-RPH3A antibody (A10247-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPH3A antigen affinity purified polyclonal antibody (Catalog # A10247-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPH3A at approximately 85 kDa. The expected band size for RPH3A is at 77 kDa.
Click image to see more details
Figure 2. IHC analysis of RPH3A using anti-RPH3A antibody (A10247-2).
RPH3A was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPH3A Antibody (A10247-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of RPH3A using anti-RPH3A antibody (A10247-2).
RPH3A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPH3A Antibody (A10247-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Rph3a (Source: Uniprot.org, NCBI)
Gene Name
Rph3a
Full Name
Rabphilin-3A
Weight
41409 MW
Alternative Names
Exophilin-1; KIAA0985exophilin-1; rabphilin 3A homolog (mouse); rabphilin; rabphilin-3A Rph3a|2900002P20Rik, AU022689, AW108370|rabphilin 3A|rabphilin-3A|Doc2 family|exophilin-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Rph3a, check out the Rph3a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Rph3a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPH3A Antibody Picoband™ (A10247-2)
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