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Product Info Summary
| SKU: | P10247 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ICC, WB |
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Product info
Product Name
Anti-Phospho-Rabphilin 3A (Ser234) Antibody
View all Rabphilin 3A Antibodies
SKU/Catalog Number
P10247
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Rabphilin 3A (Ser234) Antibody (Catalog # P10247). Tested in WB, ICC applications. This antibody reacts with Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Rabphilin 3A (Ser234) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P10247)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of rat rabphilin 3A, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P10247 is reactive to RPH3A in Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
52 kDa
Calculated molecular weight
75832 MW
Background of Rabphilin 3A
Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994; Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser-234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995; Foletti and Scheller, 2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P10247 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
ICC: 1:400
Validation Images & Assay Conditions
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Immunostaining of 14 DIV rat cortical neurons showing rabphilin when phosphorylated at Ser234 in red and PSD95 in green.
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Western blot of rat brain lysate showing specific immunolabeling of the ~82 kDa rabphilin 3A phosphorylated at Ser234.
Protein Target Info & Infographic
Gene/Protein Information For RPH3A (Source: Uniprot.org, NCBI)
Gene Name
RPH3A
Full Name
Rabphilin-3A
Weight
75832 MW
Alternative Names
Exophilin 1 antibody, Exophilin-1 antibody, Exophilin1 antibody, KIAA0985 antibody, Rabphilin 3A homolog antibody, Rabphilin-3A antibody, Rabphilin3A antibody, RP3A antibody, RP3A_HUMAN antibody, Rph 3a antibody, Rph3a antibody Rph3a|2900002P20Rik, AU022689, AW108370|rabphilin 3A|rabphilin-3A|Doc2 family|exophilin-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPH3A, check out the RPH3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPH3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Rabphilin 3A (Ser234) Antibody (P10247)
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1 Customer Q&As for Anti-Phospho-Rabphilin 3A (Ser234) Antibody
Question
We are currently using anti-Phospho-Rabphilin 3A (Ser234) antibody P10247 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
The anti-Phospho-Rabphilin 3A (Ser234) antibody (P10247) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-14
