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Product Info Summary
| SKU: | A11439 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-RPA Interacting Protein Antibody
SKU/Catalog Number
A11439
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RPA Interacting Protein Antibody (Catalog # A11439). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RPA Interacting Protein antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RPA Interacting Protein Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11439)
Host
Rabbit
Contents
RPA Interacting Protein Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RPA IP antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human RPA IP. The immunogen is located within the last 50 amino acids of RPA Interacting Protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TMIGD2 antibody is human and mouse reactive. At least three isoforms of TMIGD2 are known to exist; this antibody will detect all three. TMIGD2 antibody is predicted to not cross-react with TMIGD1.
Reactive Species
A11439 is reactive to RPAIN in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
24770 MW
Background of Rpain
Replication protein A (RPA) is a single-stranded-DNA binding protein involved in numerous eukaryotic DNA processes including replication, repair and recombination. RPA interacting protein (RPA IP) has been identified as an adapter protein that is involved in RPA nuclear import instead of the prototypical importin proteins that normally mediate nuclear import. Multiple isoforms of RPA IP are known to exist, with the longest isoform localized to the cytoplasm. Isoform 2 is sumoylated and is located in the PML nuclear body within the nucleus. It has been suggested that this isoform mediates the localization of the RPA complex into the PML nuclear body, thereby participating in RPA function in DNA metabolism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11439 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RPA Interacting Protein antibody can be used for detection of RPA Interacting Protein by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RPA Interacting Protein in Jurkat cell lysate with RPA Interacting Protein antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of RPA Interacting Protein in mouse stomach tissue with RPA Interacting Protein antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For RPAIN (Source: Uniprot.org, NCBI)
Gene Name
RPAIN
Full Name
RPA-interacting protein
Weight
24770 MW
Alternative Names
RIP, HRIP, RIP, RPA-interacting protein, hRIP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPAIN, check out the RPAIN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPAIN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPA Interacting Protein Antibody (A11439)
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