Anti-ROC2 RNF7 Antibody

RNF7 antibody

Boster Bio Anti-ROC2 RNF7 Antibody (Catalog # A05620). Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A05620
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-ROC2 RNF7 Antibody

View all RNF7 Antibodies

SKU/Catalog Number

A05620

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-ROC2 RNF7 Antibody (Catalog # A05620). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-ROC2 RNF7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05620)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05620 is reactive to RNF7 in Human

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

100124 MW

Background of RNF7

ROC2 also known as RING-box protein 2, Rbx2, RING finger protein 7, Regulator of cullins 2, CKII beta-binding protein 1, and CKBBP1, is a probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC2 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. ROC2 may play a role in protecting cells from apoptosis induced by redox agents. ROC2 has a cytoplasmic and nuclear localization and is expressed in heart, liver, skeletal muscle and pancreas tissues, and at very low levels in brain, placenta and lung. 1,10-phenanthroline induces ROC2 expression. The RING-type zinc finger domain is essential for ubiquitin ligase activity. Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A05620 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: User optimized
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

Gene/Protein Information For RNF7 (Source: Uniprot.org, NCBI)

Gene Name

RNF7

Full Name

RING-box protein 2

Weight

100124 MW

Superfamily

RING-box family

Alternative Names

ROC2, GTP binding protein Roc2, Ras like protein expressed in neurons, Ras like without CAAX 2, Ras like without CAAX protein 2, RIBA, Ric (Drosophila) like, Ric like, RIN RNF7 CKBBP1, ROC2, SAG, rbx2 ring finger protein 7 RING-box protein 2|CKII beta-binding protein 1|regulator of cullins 2|sensitive to apoptosis gene protein|sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2|zinc RING finger protein SAG

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RNF7, check out the RNF7 Infographic

RNF7 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RNF7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for A05620

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-ROC2 RNF7 Antibody?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-ROC2 RNF7 Antibody

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

2 Customer Q&As for Anti-ROC2 RNF7 Antibody

Question

We are currently using anti-ROC2 antibody A05620 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

The anti-ROC2 antibody (A05620) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-02

Question

Is this A05620 anti-ROC2 antibody reactive to the isotypes of RNF7?

Verified Customer

Verified customer

Asked: 2019-03-12

Answer

The immunogen of A05620 anti-ROC2 antibody is This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 102-113 of Human ROC2 (C-terminal) coupled to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-03-12

Order DetailsPrice
A05620

100uL

$629

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
A05620
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$629.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.