Click image to see more details
Product Info Summary
| SKU: | A08456 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GCF GCFC2 Antibody
SKU/Catalog Number
A08456
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-GCF GCFC2 Antibody (Catalog # A08456). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCF GCFC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08456)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human GCF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08456 is reactive to GCFC2 in Human, Mouse
Reconstitution
Calculated molecular weight
89385 MW
Background of GCFC2
Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. Involved in pre-mRNA splicing through regulating spliceosome C complex formation. May play a role during late-stage splicing events and turnover of excised inrons.
Kageyama R., Cell 59:815-825(1989).
Hillier L.W., Nature 434:724-731(2005).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08456 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from RAW264.7 cells, using GCF antibody A08456.
Protein Target Info & Infographic
Gene/Protein Information For GCFC2 (Source: Uniprot.org, NCBI)
Gene Name
GCFC2
Full Name
GC-rich sequence DNA-binding factor 2
Weight
89385 MW
Superfamily
GCF family
Alternative Names
GC-rich sequence DNA-binding factor 2;GC-rich sequence DNA-binding factor;Transcription factor 9;TCF-9;GCFC2;C2orf3, GCF, TCF9; GCFC2 C2orf3, DNABF, GCF, TCF9 GC-rich sequence DNA-binding factor 2 intron Large complex component GCFC2|GC binding factor|GC bindng factor|TCF-9|transcription factor 9 (binds GC-rich sequences)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCFC2, check out the GCFC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCFC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GCF GCFC2 Antibody (A08456)
Hello CJ!
No publications found for A08456
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GCF GCFC2 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-GCF GCFC2 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-GCF GCFC2 Antibody
Question
Is this A08456 anti-GCF antibody reactive to the isotypes of GCFC2?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The immunogen of A08456 anti-GCF antibody is Synthesized peptide derived from internal of human GCF. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-03
Question
Do you have a BSA free version of anti-GCF antibody A08456 available?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GCF antibody A08456 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-28
Question
We are currently using anti-GCF antibody A08456 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
The anti-GCF antibody (A08456) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-12
Question
Is a blocking peptide available for product anti-GCF antibody (A08456)?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
We do provide the blocking peptide for product anti-GCF antibody (A08456). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-GCF antibody A08456. Let me know if you need anything else.
E. Williams
Verified customer
Asked: 2017-10-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-16
Question
Will anti-GCF antibody A08456 work for WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-08-11
Answer
According to the expression profile of leukemic t-cell, GCFC2 is highly expressed in leukemic t-cell. So, it is likely that anti-GCF antibody A08456 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2017-08-11
Question
I see that the anti-GCF antibody A08456 works with WB, what is the protocol used to produce the result images on the product page?
D. Jackson
Verified customer
Asked: 2015-10-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-10-01
