Click image to see more details
Product Info Summary
| SKU: | A15126 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Gap junction beta-7 protein GJB7 Antibody
SKU/Catalog Number
A15126
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Gap junction beta-7 protein GJB7 Antibody (Catalog # A15126). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gap junction beta-7 protein GJB7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15126)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human GJB7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15126 is reactive to GJB7 in Human
Reconstitution
Calculated molecular weight
25860 MW
Background of GJB7
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell By similarity.
Willecke K., Biol. Chem. 383:725-737(2002).
Mungall A.J., Nature 425:805-811(2003).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15126 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HT-29 cells, using GJB7 antibody A15126.
Protein Target Info & Infographic
Gene/Protein Information For GJB7 (Source: Uniprot.org, NCBI)
Gene Name
GJB7
Full Name
Gap junction beta-7 protein
Weight
25860 MW
Superfamily
connexin family
Alternative Names
Gap junction beta-7 protein;Connexin-25;Cx25;GJB7;CX25; GJB7 CX25, bA136M9.1, connexin25 gap junction protein beta 7 gap junction beta-7 protein|connexin-25|gap junction protein, beta 7, 25kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GJB7, check out the GJB7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GJB7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Gap junction beta-7 protein GJB7 Antibody (A15126)
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No publications found for A15126
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Gap junction beta-7 protein GJB7 Antibody
Question
Would anti-GJB7 antibody A15126 work for WB with tongue?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
According to the expression profile of tongue, GJB7 is highly expressed in tongue. So, it is likely that anti-GJB7 antibody A15126 will work for WB with tongue.
Boster Scientific Support
Answered: 2019-08-20
Question
I see that the anti-GJB7 antibody A15126 works with WB, what is the protocol used to produce the result images on the product page?
E. Baker
Verified customer
Asked: 2019-05-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-24
Question
I have a question about product A15126, anti-GJB7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Krishna
Verified customer
Asked: 2019-02-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15126 anti-GJB7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-05
Question
Is a blocking peptide available for product anti-GJB7 antibody (A15126)?
D. Carter
Verified customer
Asked: 2018-09-11
Answer
We do provide the blocking peptide for product anti-GJB7 antibody (A15126). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-11
Question
Does A15126 anti-GJB7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Moore
Verified customer
Asked: 2018-06-21
Answer
As indicated on the product datasheet, A15126 anti-GJB7 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-21
Question
Would anti-GJB7 antibody A15126 work on goat WB with placenta?
K. Huang
Verified customer
Asked: 2013-11-18
Answer
Our lab technicians have not validated anti-GJB7 antibody A15126 on goat. You can run a BLAST between goat and the immunogen sequence of anti-GJB7 antibody A15126 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-11-18
