Anti-RNF14 Mouse Monoclonal Antibody [Clone ID: OTI1C9]

Product Name

Anti-RNF14 Mouse Monoclonal Antibody [Clone ID: OTI1C9]

View all ARA54 Antibodies

SKU/Catalog Number

M08956

Size

100 µl

Description

Boster Bio RNF14 mouse monoclonal antibody,clone OTI1C9. Catalog# M08956. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C as received.

Cite This Product

Anti-RNF14 Mouse Monoclonal Antibody [Clone ID: OTI1C9] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08956)

Host

Mouse

Contents

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

OTI1C9

Isotype

IgG2a

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-220 of human RNF14 (NP_899647) produced in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M08956 is reactive to RNF14 in Human, Mouse, Rat

Applications

M08956 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Validation Images & Assay Conditions

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF14 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF14 (1:2000).

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Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-RNF14 monoclonal antibody (1:500).

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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RNF14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min

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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RNF14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min

Gene/Protein Information For RNF14 (Source: Uniprot.org, NCBI)

Alternative Names

Androgen receptor-associated protein 54; ARA54; ARA54androgen receptor associated protein 54; EC 6.3.2.-; HFB30; HFB30E3 ubiquitin-protein ligase RNF14; ring finger protein 14FLJ26004; RNF14; Triad2 protein; TRIAD2 RNF14 ARA54, HFB30, HRIHFB2038, TRIAD2 ring finger protein 14 E3 ubiquitin-protein ligase RNF14|androgen receptor associated protein 54

For more info on RNF14, check out the RNF14 Infographic

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In this infographic, you will see the following information for RNF14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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