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Product Info Summary
| SKU: | A06347 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RND1 Antibody Picoband®
SKU/Catalog Number
A06347
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RND1 Antibody Picoband® catalog # A06347. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RND1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06347)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human RND1 recombinant protein (Position: K105-C229). Human RND1 shares 95.2% amino acid (aa) sequence identity with mouse RND1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06347 is reactive to RND1 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
26056 MW
Background of RND1
Rnd1 is a small (~21 kDa) signaling G protein (to be specific, a GTPase), and is a member of the Rndsubgroup of the Rho family of GTPases. Members of this family regulate the organization of the actin cytoskeleton in response to extracellular growth factors. A similar protein in rat interacts with a microtubule regulator to control axon extension. The RND1 gene is mapped to chromosome 12q12-q13.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06347 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Human
Positive Control
WB: mouse liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RND1 using anti-RND1 antibody (A06347).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RND1 antigen affinity purified polyclonal antibody (Catalog # A06347) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RND1 at approximately 26KD. The expected band size for RND1 is at 26KD.
Protein Target Info & Infographic
Gene/Protein Information For RND1 (Source: Uniprot.org, NCBI)
Gene Name
RND1
Full Name
Rho-related GTP-binding protein Rho6
Weight
26056 MW
Superfamily
small GTPase superfamily
Alternative Names
Rho-related GTP-binding protein Rho6;Rho family GTPase 1;Rnd1;RND1;RHO6; RND1 ARHS, RHO6, RHOS Rho family GTPase 1 rho-related GTP-binding protein Rho6|ras homolog gene family, member S
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RND1, check out the RND1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RND1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RND1 Antibody Picoband® (A06347)
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No publications found for A06347
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Customer Q&As
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7 Customer Q&As for Anti-RND1 Antibody Picoband®
Question
Will A06347 anti-RND1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
It shows on the product datasheet, A06347 anti-RND1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-14
Question
Is a blocking peptide available for product anti-RND1 antibody (A06347)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We do provide the blocking peptide for product anti-RND1 antibody (A06347). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-10
Question
I see that the anti-RND1 antibody A06347 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-06
Question
See attached the WB image, lot number and protocol we used for lung using anti-RND1 antibody A06347. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-05
Question
Would anti-RND1 antibody A06347 work on goat WB with brain?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Our lab technicians have not validated anti-RND1 antibody A06347 on goat. You can run a BLAST between goat and the immunogen sequence of anti-RND1 antibody A06347 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-27
Question
We are currently using anti-RND1 antibody A06347 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-RND1 antibody (A06347) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-RND1 antibody A06347. Let me know if you need anything else.
S. Moore
Verified customer
Asked: 2014-03-27
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-27
