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Product Info Summary
| SKU: | A06008-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GTP-binding protein Rit1 Rit1 Antibody
SKU/Catalog Number
A06008-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GTP-binding protein Rit1 Rit1 Antibody catalog # A06008-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GTP-binding protein Rit1 Rit1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06008-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RIT1. AA range:170-219
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06008-1 is reactive to RIT1 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
25145 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06008-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using Rit1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For RIT1 (Source: Uniprot.org, NCBI)
Gene Name
RIT1
Full Name
GTP-binding protein Rit1
Weight
25145 MW
Superfamily
small GTPase superfamily
Alternative Names
GTP-binding protein Rit1;Ras-like protein expressed in many tissues;Ras-like without CAAX protein 1;RIT1;RIBB, RIT, ROC1; RIT1 NS8, RIBB, RIT, ROC1 Ras like without CAAX 1 GTP-binding protein Rit1|GTP-binding protein Roc1|Ric-like, expressed in many tissues|ras-like protein expressed in many tissues|ras-like without CAAX protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIT1, check out the RIT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GTP-binding protein Rit1 Rit1 Antibody (A06008-1)
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No publications found for A06008-1
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Customer Q&As
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6 Customer Q&As for Anti-GTP-binding protein Rit1 Rit1 Antibody
Question
Do you have a BSA free version of anti-Rit1 antibody A06008-1 available?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Rit1 antibody A06008-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-16
Question
My question regarding product A06008-1, anti-Rit1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Johnson
Verified customer
Asked: 2019-12-31
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06008-1 anti-Rit1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-31
Question
We are currently using anti-Rit1 antibody A06008-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The anti-Rit1 antibody (A06008-1) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-06
Question
We are interested in to test anti-Rit1 antibody A06008-1 on mouse retina for research purposes, then I may be interested in using anti-Rit1 antibody A06008-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
The products we sell, including anti-Rit1 antibody A06008-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-23
Question
Would A06008-1 anti-Rit1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Carter
Verified customer
Asked: 2017-02-01
Answer
As indicated on the product datasheet, A06008-1 anti-Rit1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-02-01
Question
Is this A06008-1 anti-Rit1 antibody reactive to the isotypes of RIT1?
E. Jha
Verified customer
Asked: 2015-12-31
Answer
The immunogen of A06008-1 anti-Rit1 antibody is Synthesized peptide derived from the C-terminal region of human Rit1. at AA rangle: 140-220. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-12-31
