Anti-RIN1 Antibody Picoband™

Product Name

Anti-RIN1 Antibody Picoband™

View all RIN1 Antibodies

SKU/Catalog Number

A04953-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RIN1 Antibody Picoband™ catalog # A04953-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RIN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04953-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RIN1 recombinant protein (Position: A76-N669).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04953-1 is reactive to RIN1 in Human

Applications

A04953-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

90 kDa

Calculated molecular weight

45468 MW

Background of RIN1

Ras and Rab interactor 1 is a protein that in humans is encoded by the RIN1 gene. Predicted to enable small GTPase binding activity. Predicted to be involved in endocytosis; regulation of catalytic activity; and signal transduction. Predicted to act upstream of or within associative learning; memory; and negative regulation of synaptic plasticity. Located in cytoplasm and plasma membrane.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RIN1 using anti-RIN1 antibody (A04953-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIN1 antigen affinity purified polyclonal antibody (Catalog # A04953-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIN1 at approximately 90 kDa. The expected band size for RIN1 is at 84 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-RIN1 antibody (A04953-1).
Overlay histogram showing HepG2 cells stained with A04953-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIN1 Antibody (A04953-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For RIN1 (Source: Uniprot.org, NCBI)

Alternative Names

Ras and Rab interactor 1; ras inhibitor 1; Ras inhibitor JC99; ras inhibitor RIN1; Ras interaction/interference protein 1 RIN1 Ras and Rab interactor 1 ras and Rab interactor 1|ras inhibitor 1|ras inhibitor JC99|ras inhibitor RIN1|ras interaction/interference protein 1

For more info on RIN1, check out the RIN1 Infographic

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In this infographic, you will see the following information for RIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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