Product Info Summary
SKU: | A03774 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-FUT3/Fuct Iii Antibody
SKU/Catalog Number
A03774
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-FUT3/Fuct Iii Antibody (Catalog # A03774). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FUT3/Fuct Iii Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03774)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human FUT3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03774 is reactive to FUT3 in Human
Reconstitution
Calculated molecular weight
42117 MW
Background of FUT3
May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.
Kukowska-Latallo J.F., Genes Dev. 4:1288-1303(1990).
Cameron H.S., J. Biol. Chem. 270:20112-20122(1995).
Rahim I., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03774 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells and Jurkat cells, using MRPS7 antibody A03774.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MRPS7 antibody A03774.
Protein Target Info & Infographic
Gene/Protein Information For FUT3 (Source: Uniprot.org, NCBI)
Gene Name
FUT3
Full Name
Galactoside 3(4)-L-fucosyltransferase
Weight
42117 MW
Superfamily
glycosyltransferase 10 family
Alternative Names
Galactoside 3 (4)-L-fucosyltransferase;2.4.1.65;Blood group Lewis alpha-4-fucosyltransferase;Lewis FT;Fucosyltransferase 3;Fucosyltransferase III;FucT-III;FUT3;FT3B, LE; FUT3 CD174, FT3B, FucT-III, LE, Les fucosyltransferase 3 (Lewis blood group) 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3|Lewis FT|alpha-(1,3/1,4)-fucosyltransferase|alpha-3-fucosyltransferase FUT3|blood group Lewis alpha-4-fucosyltransferase|fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)|fucosyltransferase III|galactoside 3(4)-L-fucosyltransferase|truncated fucosyltransferase 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FUT3, check out the FUT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FUT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FUT3/Fuct Iii Antibody (A03774)
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No publications found for A03774
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-FUT3/Fuct Iii Antibody
Question
Is this A03774 anti-FUT3/Fuct Iii antibody reactive to the isotypes of FUT3?
Verified Customer
Verified customer
Asked: 2019-03-22
Answer
The immunogen of A03774 anti-FUT3/Fuct Iii antibody is Synthesized peptide derived from internal of human FUT3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-22
Question
Here is the WB image, lot number and protocol we used for lower esophagus mucosa using anti-FUT3/Fuct Iii antibody A03774. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-02
Question
Is there a BSA free version of anti-FUT3/Fuct Iii antibody A03774 available?
Verified Customer
Verified customer
Asked: 2018-04-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FUT3/Fuct Iii antibody A03774 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-23
Question
We are currently using anti-FUT3/Fuct Iii antibody A03774 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
K. Gonzalez
Verified customer
Asked: 2013-10-07
Answer
The anti-FUT3/Fuct Iii antibody (A03774) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-07