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Product Info Summary
| SKU: | M00083-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Vertebrates |
| Host: | Mouse |
| Application: | ELISA, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Rhodopsin Monoclonal Antibody
SKU/Catalog Number
M00083-2
Size
100ug
Form
liquid
Description
Boster Bio Anti-Rhodopsin Monoclonal Antibody catalog # M00083-2. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rhodopsin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00083-2)
Host
Mouse
Contents
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
1D4
Isotype
IgG1
Immunogen
Bovine Rhodopsin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Reactive Species
M00083-2 is reactive to RHO in Vertebrates
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
19400 MW
Background of Rhodopsin
Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00083-2 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
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Figure 1. IHC analysis of RHO using anti-RHO antibody (M00083-2).
RHO was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RHO Antibody (M00083-2) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 2. Western blot analysis of RHO using anti-RHO antibody (M00083-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHO antigen affinity purified polyclonal antibody (Catalog # M00083-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for RHO.
Protein Target Info & Infographic
Gene/Protein Information For RHO (Source: Uniprot.org, NCBI)
Gene Name
RHO
Full Name
Rhodopsin
Weight
19400 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
OPN2, opsd, opsin 2, opsin 2 rod pigment, opsin2, RHO, RP4, MGC138309, Retinitis Pigmentosa 4 RHO CSNBAD1, OPN2, RP4 rhodopsin rhodopsin|opsin 2, rod pigment|opsin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHO, check out the RHO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rhodopsin Monoclonal Antibody (M00083-2)
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