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Product Info Summary
| SKU: | P30435 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-Phospho Rhodopsin (Ser334) Antibody
SKU/Catalog Number
P30435
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho Rhodopsin (Ser334) Antibody (Catalog# P30435). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho Rhodopsin (Ser334) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30435)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human Rhodopsin around the phosphorylation site of Ser334. Immunogen sequence location: 299-348
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P30435 is reactive to RHO in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
38893 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30435 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain, using Rhodopsin (Phospho-Ser334) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For RHO (Source: Uniprot.org, NCBI)
Gene Name
RHO
Full Name
Rhodopsin
Weight
38893 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
RHO;OPN2;Rhodopsin;Opsin-2 RHO CSNBAD1, OPN2, RP4 rhodopsin rhodopsin|opsin 2, rod pigment|opsin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHO, check out the RHO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho Rhodopsin (Ser334) Antibody (P30435)
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No publications found for P30435
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Customer Q&As
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5 Customer Q&As for Anti-Phospho Rhodopsin (Ser334) Antibody
Question
Would anti-Phospho Rhodopsin (Ser334) antibody P30435 work on pig IHC with retina?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Our lab technicians have not tested anti-Phospho Rhodopsin (Ser334) antibody P30435 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Phospho Rhodopsin (Ser334) antibody P30435 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig retina in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-23
Question
I see that the anti-Phospho Rhodopsin (Ser334) antibody P30435 works with IHC, what is the protocol used to produce the result images on the product page?
M. Zhang
Verified customer
Asked: 2019-07-10
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-10
Question
I am interested in to test anti-Phospho Rhodopsin (Ser334) antibody P30435 on human retina for research purposes, then I may be interested in using anti-Phospho Rhodopsin (Ser334) antibody P30435 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
The products we sell, including anti-Phospho Rhodopsin (Ser334) antibody P30435, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-09
Question
We are currently using anti-Phospho Rhodopsin (Ser334) antibody P30435 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
The anti-Phospho Rhodopsin (Ser334) antibody (P30435) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-17
Question
Would anti-Phospho Rhodopsin (Ser334) antibody P30435 work for IHC with retina?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
According to the expression profile of retina, RHO is highly expressed in retina. So, it is likely that anti-Phospho Rhodopsin (Ser334) antibody P30435 will work for IHC with retina.
Boster Scientific Support
Answered: 2018-12-26
