Click image to see more details
Product Info Summary
| SKU: | M00083-1 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Mammals, Amphibians, Fish, Shark, Avian |
| Host: | Mouse |
| Application: | ELISA, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Rhodopsin Monoclonal Antibody
SKU/Catalog Number
M00083-1
Size
100ug
Form
liquid
Description
Boster Bio Anti-Rhodopsin Monoclonal Antibody catalog # M00083-1. Tested in ELISA, IP, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rhodopsin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00083-1)
Host
Mouse
Contents
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
4D2
Isotype
IgG1
Immunogen
Bovine Rhodopsin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Reactive Species
M00083-1 is reactive to RHO in Mammals, Amphibians, Fish, Shark, Avian
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
19400 MW
Background of Rhodopsin
Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00083-1 is guaranteed for ELISA, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of RHO using anti-RHO antibody (M00083-1).
RHO was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RHO Antibody (M00083-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For RHO (Source: Uniprot.org, NCBI)
Gene Name
RHO
Full Name
Rhodopsin
Weight
19400 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
OPN2, opsd, opsin 2, opsin 2 rod pigment, opsin2, RHO, RP4, MGC138309, Retinitis Pigmentosa 4 RHO CSNBAD1, OPN2, RP4 rhodopsin rhodopsin|opsin 2, rod pigment|opsin-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHO, check out the RHO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rhodopsin Monoclonal Antibody (M00083-1)
Hello CJ!
No publications found for M00083-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Rhodopsin Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Rhodopsin Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
