Anti-DNA-binding protein RFX5 RFX5 Antibody

Product Name

Anti-DNA-binding protein RFX5 RFX5 Antibody

View all RFX5 Antibodies

SKU/Catalog Number

A05527

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-DNA-binding protein RFX5 RFX5 Antibody (Catalog # A05527). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-DNA-binding protein RFX5 RFX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05527)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A05527 is reactive to RFX5 in Human

Applications

A05527 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

11532 MW

Background of RFX5

DNA-binding protein RFX5 activates transcription from class II MHC promoters and recognizes X-boxes. It mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. RFX5 may be associated with Bare Lymphocyte Syndrome Type li Complementation group C and Mhc Class Ii Deficiency. Anti-RFX5 Antibody is useful for researchers interested in Transcription factors, Tuberculosis, and Allograft rejection.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
WB: 1:500 - 1:3,000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RFX5 using anti-RFX5 antibody (A05527).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX5 antigen affinity purified polyclonal antibody (Catalog # A05527) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for RFX5.

Gene/Protein Information For RFX5 (Source: Uniprot.org, NCBI)

Alternative Names

DNA-binding protein RFX5; Regulatory factor X 5; regulatory factor X, 5 (influences HLA class II expression) RFX5 regulatory factor X5 DNA-binding protein RFX5|regulatory factor X 5|regulatory factor X, 5 (influences HLA class II expression)

For more info on RFX5, check out the RFX5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RFX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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