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Product Info Summary
| SKU: | A05527 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DNA-binding protein RFX5 RFX5 Antibody
SKU/Catalog Number
A05527
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-DNA-binding protein RFX5 RFX5 Antibody (Catalog # A05527). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-DNA-binding protein RFX5 RFX5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05527)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05527 is reactive to RFX5 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
11532 MW
Background of RFX5
DNA-binding protein RFX5 activates transcription from class II MHC promoters and recognizes X-boxes. It mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. RFX5 may be associated with Bare Lymphocyte Syndrome Type li Complementation group C and Mhc Class Ii Deficiency. Anti-RFX5 Antibody is useful for researchers interested in Transcription factors, Tuberculosis, and Allograft rejection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05527 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:25,000
WB: 1:500 - 1:3,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RFX5 using anti-RFX5 antibody (A05527).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFX5 antigen affinity purified polyclonal antibody (Catalog # A05527) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for RFX5.
Protein Target Info & Infographic
Gene/Protein Information For RFX5 (Source: Uniprot.org, NCBI)
Gene Name
RFX5
Full Name
DNA-binding protein RFX5
Weight
11532 MW
Superfamily
RFX family
Alternative Names
RFX5, DNA binding protein RFX5, Influences HLA class II expression, Regulatory factor X 5, Regulatory factor X subunit 5 RFX5 regulatory factor X5 DNA-binding protein RFX5|regulatory factor X 5|regulatory factor X, 5 (influences HLA class II expression)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RFX5, check out the RFX5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RFX5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA-binding protein RFX5 RFX5 Antibody (A05527)
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No publications found for A05527
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Customer Q&As
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4 Customer Q&As for Anti-DNA-binding protein RFX5 RFX5 Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-RFX5 antibody A05527. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-12
Question
We are currently using anti-RFX5 antibody A05527 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
O. Banerjee
Verified customer
Asked: 2015-09-02
Answer
The anti-RFX5 antibody (A05527) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-09-02
Question
Do you have a BSA free version of anti-RFX5 antibody A05527 available?
V. Carter
Verified customer
Asked: 2015-04-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RFX5 antibody A05527 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-04-15
Question
See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-RFX5 antibody A05527. Please let me know if you require anything else.
S. Williams
Verified customer
Asked: 2014-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-06-20
