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Product Info Summary
| SKU: | A06128 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NF-YC Antibody
SKU/Catalog Number
A06128
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NF-YC Antibody catalog # A06128. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF-YC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06128)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NFYC. AA range:1-50
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06128 is reactive to NFYC in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
50302 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06128 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HeLa cells using NF-YC Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using NF-YC Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For NFYC (Source: Uniprot.org, NCBI)
Gene Name
NFYC
Full Name
Nuclear transcription factor Y subunit gamma
Weight
50302 MW
Superfamily
NFYC/HAP5 subunit family
Alternative Names
Nuclear transcription factor Y subunit gamma;CAAT box DNA-binding protein subunit C;Nuclear transcription factor Y subunit C;NF-YC;Transactivator HSM-1/2;NFYC; NFYC CBF-C, CBFC, H1TF2A, HAP5, HSM, NF-YC nuclear transcription factor Y subunit gamma nuclear transcription factor Y subunit gamma|CAAT box DNA-binding protein subunit C|CCAAT binding factor subunit C|CCAAT transcription binding factor subunit gamma|histone H1 transcription factor large subunit 2A|nuclear transcription factor Y subunit C|nuclear transcription factor Y, gamma|transactivator HSM-1|transactivator HSM-1/2|transcription factor NF-Y, C subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFYC, check out the NFYC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFYC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NF-YC Antibody (A06128)
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No publications found for A06128
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Customer Q&As
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7 Customer Q&As for Anti-NF-YC Antibody
Question
I have attached the WB image, lot number and protocol we used for brain using anti-NF-YC antibody A06128. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
Is a blocking peptide available for product anti-NF-YC antibody (A06128)?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
We do provide the blocking peptide for product anti-NF-YC antibody (A06128). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-14
Question
Can you help my question with product A06128, anti-NF-YC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06128 anti-NF-YC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-26
Question
Will anti-NF-YC antibody A06128 work on goat WB with epithelium of mammary gland?
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
Our lab technicians have not tested anti-NF-YC antibody A06128 on goat. You can run a BLAST between goat and the immunogen sequence of anti-NF-YC antibody A06128 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat epithelium of mammary gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-27
Question
We are currently using anti-NF-YC antibody A06128 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
The anti-NF-YC antibody (A06128) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-09
Question
I am interested in to test anti-NF-YC antibody A06128 on mouse brain for research purposes, then I may be interested in using anti-NF-YC antibody A06128 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
The products we sell, including anti-NF-YC antibody A06128, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-09
Question
Does A06128 anti-NF-YC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Carter
Verified customer
Asked: 2017-12-04
Answer
As indicated on the product datasheet, A06128 anti-NF-YC antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-04
