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Product Info Summary
| SKU: | M00293 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Ret Rabbit Monoclonal Antibody
SKU/Catalog Number
M00293
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ret Rabbit Monoclonal Antibody catalog # M00293. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ret Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00293)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FIF-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ret
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00293 is reactive to RET in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
3 kDa
Calculated molecular weight
124319 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00293 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: SH-SY5Y cell
Validation Images & Assay Conditions
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Western blot analysis of Ret expression in SH-SY5Y cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RET (Source: Uniprot.org, NCBI)
Gene Name
RET
Full Name
Proto-oncogene tyrosine-protein kinase receptor Ret
Weight
124319 MW
Superfamily
protein kinase superfamily
Alternative Names
Proto-oncogene tyrosine-protein kinase receptor Ret;2.7.10.1;Cadherin family member 12;Proto-oncogene c-Ret;Soluble RET kinase fragment;Extracellular cell-membrane anchored RET cadherin 120 kDa fragment;RET;CDHF12, CDHR16, PTC, RET51; RET CDHF12, CDHR16, HSCR1, MEN2A, MEN2B, MTC1, PTC-ELE1, RET ret proto-oncogene proto-oncogene tyrosine-protein kinase receptor Ret|RET receptor tyrosine kinase|cadherin family member 12|cadherin-related family member 16|proto-oncogene c-Ret|rearranged during transfection|ret proto-oncogene (multiple endocrine neoplasia and medullary thyroid carcinoma 1, Hirschsprung disease)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RET, check out the RET Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RET: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ret Rabbit Monoclonal Antibody (M00293)
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No publications found for M00293
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Ret Rabbit Monoclonal Antibody
Question
We have been able to see staining in rat prostate. Are there any suggestions? Is anti-Ret Rabbit Monoclonal antibody supposed to stain prostate positively?
Verified Customer
Verified customer
Asked: 2018-04-10
Answer
From literature prostate does express RET. From Uniprot.org, RET is expressed in dorsal root ganglion, prostate, brain, fibroblast, thyroid papillary carcinoma, blood, leukocyte, peripheral blood leukocyte, among other tissues. Regarding which tissues have RET expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7633441
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2734021
Leukocyte, Pubmed ID: 7581377
Peripheral blood leukocyte, Pubmed ID: 9360560
Prostate, Pubmed ID: 14702039
Thyroid papillary carcinoma, Pubmed ID: 2406025
Boster Scientific Support
Answered: 2018-04-10
Question
We are currently using anti-Ret Rabbit Monoclonal antibody M00293 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
J. Anderson
Verified customer
Asked: 2016-04-11
Answer
The anti-Ret Rabbit Monoclonal antibody (M00293) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-11
Question
My team were satisfied with the WB result of your anti-Ret Rabbit Monoclonal antibody. However we have observed positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is RET supposed to be expressed?
E. Kulkarni
Verified customer
Asked: 2016-02-25
Answer
From literature, blood does express RET. Generally RET expresses in cell membrane. Regarding which tissues have RET expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7633441
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2734021
Leukocyte, Pubmed ID: 7581377
Peripheral blood leukocyte, Pubmed ID: 9360560
Prostate, Pubmed ID: 14702039
Thyroid papillary carcinoma, Pubmed ID: 2406025
Boster Scientific Support
Answered: 2016-02-25
Question
We ordered your anti-Ret Rabbit Monoclonal antibody for WB on leukocyte last year. I am using rat, and We are going to use the antibody for IP next. I am interested in examining leukocyte as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
D. Mitchell
Verified customer
Asked: 2015-01-02
Answer
I looked at the website and datasheets of our anti-Ret Rabbit Monoclonal antibody and I see that M00293 has been validated on rat in both WB and IP. Thus M00293 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-01-02
