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Product Info Summary
| SKU: | A05900-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Regucalcin/RGN Antibody Picoband®
View all Regucalcin Antibodies
SKU/Catalog Number
A05900-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Regucalcin/RGN Antibody Picoband® catalog # A05900-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Regucalcin/RGN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05900-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Regucalcin, which shares 87.5% and 84.4% amino acid (aa) sequence identity with mouse and rat Regucalcin, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05900-1 is reactive to RGN in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa, 43 kDa
Calculated molecular weight
33.253kDa
Background of Regucalcin
Regucalcin is a protein that in humans is encoded by the RGN gene. The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05900-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Positive Control
WB: rat liver tissue, mouse liver tissue, human SMMC-7721 whole cell
IHC: mouse liver tissue, rat kidney tissue, rat liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Regucalcin using anti-Regucalcin antibody (A05900-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: human SMMC-7721 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Regucalcin antigen affinity purified polyclonal antibody (Catalog # A05900-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Regucalcin at approximately 33, 43KD. The expected band size for Regucalcin is at 33KD.
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Figure 2. IHC analysis of Regucalcin using anti-Regucalcin antibody (A05900-1).
Regucalcin was detected in paraffin-embedded section of mouse liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Regucalcin Antibody (A05900-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Regucalcin using anti-Regucalcin antibody (A05900-1).
Regucalcin was detected in paraffin-embedded section of rat kidney tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Regucalcin Antibody (A05900-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Regucalcin using anti-Regucalcin antibody (A05900-1).
Regucalcin was detected in paraffin-embedded section of rat liver tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Regucalcin Antibody (A05900-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For RGN (Source: Uniprot.org, NCBI)
Gene Name
RGN
Full Name
Regucalcin
Weight
33.253kDa
Superfamily
SMP-30/CGR1 family
Alternative Names
Regucalcin; RC; Gluconolactonase; GNL; Senescence marker protein 30; SMP-30; RGN; SMP30 RGN GNL, HEL-S-41, RC, SMP30 regucalcin regucalcin|epididymis secretory protein Li 41|gluconolactonase|senescence marker protein 30
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RGN, check out the RGN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RGN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Regucalcin/RGN Antibody Picoband® (A05900-1)
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Customer Q&As
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4 Customer Q&As for Anti-Regucalcin/RGN Antibody Picoband®
Question
I see that the anti-Regucalcin/RGN antibody A05900-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-19
Question
Would anti-Regucalcin/RGN antibody A05900-1 work on primate WB with liver?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
Our lab technicians have not validated anti-Regucalcin/RGN antibody A05900-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Regucalcin/RGN antibody A05900-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-08
Question
We are currently using anti-Regucalcin/RGN antibody A05900-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
The anti-Regucalcin/RGN antibody (A05900-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-23
Question
We want to test anti-Regucalcin/RGN antibody A05900-1 on human liver for research purposes, then I may be interested in using anti-Regucalcin/RGN antibody A05900-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
The products we sell, including anti-Regucalcin/RGN antibody A05900-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-17
