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Product Info Summary
| SKU: | M00099-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-Ras NRAS Rabbit Monoclonal Antibody
SKU/Catalog Number
M00099-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Ras NRAS Rabbit Monoclonal Antibody catalog # M00099-2. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Ras NRAS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00099-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DBC-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Ras
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00099-2 is reactive to NRAS in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
21 kDa
Calculated molecular weight
21229 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00099-2 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:10000-1:20000
IP 1:20
FC 1:50
Positive Control
WB: C6 cell, Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of Ras expression in (1) C6 cell lysate; (2) Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NRAS (Source: Uniprot.org, NCBI)
Gene Name
NRAS
Full Name
GTPase NRas
Weight
21229 MW
Superfamily
small GTPase superfamily
Alternative Names
GTPase NRas;Transforming protein N-Ras;NRAS;HRAS1; NRAS ALPS4, CMNS, N-ras, NCMS1, NS6, NRAS NRAS proto-oncogene, GTPase GTPase NRas|N-ras protein part 4|neuroblastoma RAS viral (v-ras) oncogene homolog|neuroblastoma RAS viral oncogene homolog|transforming protein N-Ras|v-ras neuroblastoma RAS viral oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NRAS, check out the NRAS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NRAS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ras NRAS Rabbit Monoclonal Antibody (M00099-2)
Hello CJ!
M00099-2 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huanyue Cui,Xueying Liu,Jin Zhang,Ke Zhang,Dahong Yao,Shi Dong,Shushu Feng,Lu Yang, Yuyao Li,Hangyu Wang,Jian Huang,Jinhui Wang,"Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways",Evidence-Based Complementary and Alternative Medicine,vol. 2021,Article ID 6103158,18 pages,2021.https://doi.org/10.1155/2021/6103158
Species: Human,Rat
M00099-2 usage in article: APP:WB, SAMPLE:LUNG TISSUE, DILUTION:1:300
Li M, Liu W, Zhu Yf, Chen Yl, Zhang Bz, Wang R. Acta Oncol. 2006;45(8):1115-9. Correlation Of Cox-2 And K-Ras Expression To Clinical Outcome In Gastric Cancer.
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Customer Q&As
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1 Customer Q&As for Anti-Ras NRAS Rabbit Monoclonal Antibody
Question
We are currently using anti-Ras Rabbit Monoclonal antibody M00099-2 for rat tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The anti-Ras Rabbit Monoclonal antibody (M00099-2) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-21
